A method for increasing the amount of pollen collection of wild sugarcane resources
A technology of pollen collection and wild species, applied in botany equipment and methods, plant genetic improvement, application, etc., can solve the problems of utilization, lack of uniformity in flowering period, limitation of sugarcane wild species resources, in-depth research on the utilization of sugarcane distant hybridization breeding, etc. question
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Embodiment 1
[0026] Such as figure 1 shown
[0027] A method for improving the amount of pollen collection of sugarcane wild species resources, comprising the following steps:
[0028] Choose a large flower pot with good drainage and ventilation, and fill the flower pot with soil without impurities to 1 / 2 of the total volume;
[0029] Excavate the materials of wild species, such as the fine-grained shoudoumi and scorpion grass, which have the corresponding target traits (disease resistance, insect resistance or stress tolerance) and normal flowering and loose powder, from the resource garden. It is required to have tiller buds and some old roots, and cut off All the leaves of the material are planted in the flowerpot and further covered with soil to 3 / 4 of the total volume of the flowerpot. According to the amount of pollen required, the same material can be planted in 10-30 pots, watered thoroughly and covered with a transparent plastic film. Cultivate in a greenhouse at 28-32°C to let ...
Embodiment 2
[0035] The Sugarcane Research Institute of Yunnan Agricultural University has preserved nearly 1,000 copies of wild sugarcane germplasm resources collected at home and abroad. In recent years, the pollen of excellent clones has been collected for use in distant hybridization of sugarcane. There are fewer tiller stems germinated, and due to the limitation of natural conditions (the flowering period of each tiller stem is not concentrated, the pollen amount is low at low temperature, and the pollen is blown off by strong wind), each material can collect up to 0.2mL of pollen per year, which cannot meet the requirements for 1 cultivated sugarcane flower panicle carries out hybrid pollination (1 flower panicle generally needs 4.5mL pollen to be able to fully complete pollination); Use the method described in the present invention to begin to cultivate a part of hand-cut material in 2015 ( figure 2 ) and collected pollen, 30 pots were cultivated, and more than 30 tubes of pollen ( ...
Embodiment 3
[0037] Application of a method for increasing pollen collection of sugarcane wild species resources in increasing the pollen collection of close relative wild species resources of tropical crops;
[0038] The method can increase the amount of pollen collection of wild relative resources of other tropical crops, and is suitable for use by other tropical crops.
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