A method for improving hybrid seed production efficiency of self-incompatibility gramineous plants

Abstract

The invention belongs to the field of agricultural plant breeding and discloses a method for increasing the hybrid seed production efficiency of self-incompatibility gramineous plants. The method includes the steps of firstly, preparing materials, to be more specific, planting Chinese silvergrass seedlings or rhizomes in pots; secondly, culturing plants, to be more specific, culturing the pot-cultured Chinese silvergrass under 24 DEG C and in light for 16 hours / under 18 DEG C and in darkness for 8 hours, watering once each day until the soil is completely wetted, and supplementing a Hoagland plant nutrient solution three times every week; thirdly, pruning the plants, to be more specific, timely cutting off tillers after flowering each day in the flowering period to promote the plants to constantly growth new tillers and achieve earing and flowering, cutting of the tillers after flowering, and repeating to achieve annual flowering; fourthly, using dark induction to induce inflorescencespikelet to disperse pollen, to be more specific, arranging inflorescence about to enter the vigorous flowering period, and inducing the plants under room temperature in complete darkness for 8-12 hours; fifthly, collecting pollen, to be more specific, shaking the inflorescence to collect the pollen after most spikelet on the inflorescence exposes anther after the dark induction. The method is simple, efficient and beneficial to the efficiency of Chinese silvergrass hybrid breeding and genetic researches.

Description

technical field [0001] The invention belongs to the field of agricultural plant breeding, and in particular relates to a method for improving the hybrid seed production efficiency of self-incompatibility gramineous plants. Background technique [0002] Miscanthus (Miscanthus) is an important perennial C4 gramineous energy grass and forage grass. It has the characteristics of high biomass yield, wide adaptability, and strong stress resistance. It is naturally distributed in East Asia and Southeast Asia. Miscanthus species mainly include Miscanthus (M.sinensis), Miscanthus awns (M.floridulus), Grass (M.sacchariflorus), Nandi (M.lutarioriparius) and so on. Miscanthus plants usually have dozens to hundreds of tillers, and each tiller has a terminal large panicle with a length of 15-40 cm. [0003] In the state of natural growth, Miscanthus plants only have one flowering period per year, and the flowering period of different species of Miscanthus is quite different. The flowerin...

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Problems solved by technology

On the other hand, the heading and flowering of different reproductive tillers on the same Miscanthus plant are not synchronous, and the flowering time of the whole plant can generally last for 7-10 days. The spikelet flowering and loose powder in different parts of the same panicle are also gradual, located in the inflorescence The spikelets in the middle and upper parts are the first to mature and release pollen, and then bloom in batches at the upper and lower ends of the inflorescence until all the spikelets on the inflorescence are pollinated. It takes about 5-7 days for all spikelets on the entire inflorescence to complete flowering and pollen Time, it can be seen that the number of spikelets that open and the amount of pollen released by Miscanthus plants are very limited during the flowering period. Therefore, in sexual hybridization, parents often encounter the problem that the parents do not meet during the flowering and pollination period, which greatly affects hybrid seed production. Yield and efficiency cannot meet the requirements of carrying out relevant genetic research or large-scale hybrid seed production in production, which is one of the bottlenecks restricting the development of Miscanthus hybrid breeding and genetic research

[0004] In crop cross-breeding, the main method to overcome the non-meeting at flowering stage is to collect and preserve the pollen of the male parent in vitro, but our research shows that the vitality of the isolated pollen of Miscanthus can only be maintained for about 30 minutes, and it can be stored in vitro for a long time. extremely difficult

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