Preparation method and application of a kind of fruit and vegetable preservative
A fruit and vegetable fresh-keeping agent and fruit and vegetable technology, applied in the field of fruit and vegetable preservation, can solve problems such as odor, increased environmental humidity, and browning of fruit peels
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[0010] The first aspect of the present disclosure provides a method for preparing a fresh-keeping agent for fruits and vegetables, the preparation method comprising the following steps: S1. mixing rice bran with phosphate buffer solution, and then performing homogenization treatment to obtain a homogenized rice bran mixture; S2. Add pectinase, cellulase, sodium glutamate and vitamin B6 to the homogenized rice bran mixture, mix evenly and perform enzymatic reaction, and then perform inactivation treatment to inactivate pectinase and fiber enzyme to obtain the mixed solution after enzyme inactivation; S3. adjust the pH value of the mixed solution after the enzyme inactivation to 2-3, and then perform ultrasonic treatment to obtain the mixed solution after ultrasonic treatment; S4. The pH value of the treated mixed solution is adjusted to 5-5.5, then mixed with ethanol to precipitate and remove the precipitate, and the supernatant is filtered through a 1KD ultrafiltration membrane...
Embodiment 1
[0023] Mix 10 g of rice bran with 80 mL of phosphate buffer (pH 5.6) with a concentration of 1 mol / L, and then perform homogenization at a rate of 25,000 r / min for 5 minutes to obtain a homogenized rice bran mixture; Mix the rice bran mixture with 0.2g pectinase (500U / mg), 0.2g cellulase (400U / mg), 0.74g sodium glutamate and 0.05g vitamin B6, react at 45°C for 4 hours, and then Keep at 100°C for 5 minutes to inactivate pectinase and cellulase to obtain an enzyme-inactivated mixed solution; add 2mol / L hydrochloric acid to adjust the pH value of the enzyme-inactivated mixed solution to 2, and then ultrasonically treat it. The frequency of ultrasonic treatment is 80kHz, the power is 300W, the time is 30min, and the ultrasonic temperature is 60°C to obtain the mixed solution after ultrasonic treatment; add 1mol / L sodium hydroxide solution to adjust the pH value of the mixed solution after ultrasonic treatment to 5.4, Then add 54mL of absolute ethanol and mix, centrifuge at 3000r / m...
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