Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Digital PCR concentration calculation method

A calculation method and concentration technology, applied in the direction of calculation, electrical digital data processing, special data processing applications, etc., can solve problems such as measurement accuracy cannot be guaranteed, and achieve the effect of broadening the concentration detection range and improving accuracy

Active Publication Date: 2018-01-23
领航医学科技(深圳)有限公司
View PDF11 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Quantitative measurement of gene concentration by digital PCR based on Poisson distribution can have very high accuracy, but the measurement accuracy cannot be guaranteed for the dilution in the dynamic range of unknown samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Digital PCR concentration calculation method
  • Digital PCR concentration calculation method
  • Digital PCR concentration calculation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] Based on the real-time cluster concentration calculation method, multi-reaction point detection is adopted, and the reaction points are monitored in real time, and data detection is performed in each reaction cycle. The data expression of the detection reaction point can be some physical or chemical quantities that can be quantified, such as light intensity, number of molecules, number of nucleic acids, number of proteins, etc. with the number of expressed molecules or single nucleic acid or protein. This detection is a dynamic detection, and the reaction point data detection is carried out from the beginning of the reaction to the end of the reaction. The multi-cycle detection data of each reaction point should be stored correspondingly, and then draw the amplification curve diagram of the reaction point after the reaction amplification is completed. Figure 1 Generally, the fluorescence amplification curve is mainly used.

[0020] When the difference in the number of...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a digital PCR concentration calculation method, which determines and calculates the initial concentration of a reactant according to the amplification cycleCt value of the real-time fluorescence quantitative curve in the PCR amplification process combined with the real-time clustering method. The fluorescence threshold R11 has an intersection with the amplification curve ofeach positive reaction unit during exponential growth, and the intersection corresponds to the corresponding amplification cycle value Cti. The digital PCR concentration calculation method specifically includes the following steps: clustering according to the Cti, obtaining k clusters, wherein the central value of each cluster is M1, M2, ..., Mk in descending order; calculating that the number ofthe Cti contained in each cluster is Sj, calculating the number of target genes in each cluster by an Mj and the Sj, adding up the number of target genes in each cluster, finally obtaining the concentration value of the initial target genes.

Description

technical field [0001] The invention relates to a digital PCR concentration calculation method. Background technique [0002] Digital PCR is to evenly distribute the fluorescence quantitative reaction system into a large number of tiny reaction units, each of which does not contain or contains one or more target gene fragments. After the amplification, the fragments containing the target gene generate a positive detection signal, and those that do not contain the target gene do not generate a detection signal. The ratio of the number of positive reaction units determined by the end-point fluorescence signal to the total reaction units is calculated by statistical methods. The copy number of the target gene in the original sample. [0003] The quantitative measurement of gene concentration by digital PCR based on Poisson distribution can have very high accuracy, but cannot guarantee the measurement accuracy under the dilution degree in the dynamic range of unknown samples. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/24
Inventor 程标
Owner 领航医学科技(深圳)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com