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Cosmetic containing spider silk hydrolysate and preparation method thereof

A technology for cosmetics and spider silk is applied in the field of cosmetics containing spider silk hydrolyzate and its preparation, and achieves the effects of low energy consumption, low investment and high operational safety factor

Active Publication Date: 2018-01-19
HAINAN ZHUWANG PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented method involves making an eye drop containing various ingredients such as peptides or proteins from natural sources like fish scales (the protein called histidase). These components help make eyes look better by giving them their own unique texture when applied on keratin materials foundations.

Problems solved by technology

This patented technical problem addressed in this patent relates to maintaining proper hydration levels on skins due to environmental factors like sunlight exposure, heat stress, poor circulations caused by lifestyles such as exercise, smoking, eating too much food, excessive perspiration, wrinkling, bumpy appearance, etc., while providing effective treatments against various diseases including inflammation, oxynanese shortages, deteriorating conditions associated therewith, chronoaging signs, damage to human body tissues, and decreased physical fitness.

Method used

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  • Cosmetic containing spider silk hydrolysate and preparation method thereof
  • Cosmetic containing spider silk hydrolysate and preparation method thereof
  • Cosmetic containing spider silk hydrolysate and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of spider silk hydrolyzate

[0051] The hydrolysis process of the present embodiment spider silk hydrolyzate is:

[0052] (1) Pretreatment: Take 2g of spider silk, rinse it with purified water to remove impurities, and put it in an agate ball mill for crushing until the spider silk has a flocculent or powdery growth length of <1mm.

[0053] (2) Hydrolysis: Put the pulverized spider silk into a 1L glass container, add 200mL of 20g / L sodium hydroxide solution, and hydrolyze for 5h at a temperature of 80°C.

[0054] (3) Centrifugation: Centrifuge the hydrolyzate at a rotational speed of 15000r / min for 20min.

[0055] (4) Collection: Collect the centrifuged hydrolyzate into a glass container.

[0056] (5) Hydrolysis: hydrolyze the unhydrolyzed spider silk again, the method is the same as (2).

[0057] (6) Neutralization: neutralize with 1mol / L hydrochloric acid solution to pH 4.5.

[0058] (7) Filtration: filter the neutralized hydrolyzate through ...

Embodiment 2

[0060] Example 2 Preparation of spider silk hydrolyzate

[0061] The hydrolysis process of the present embodiment spider silk hydrolyzate is:

[0062] (1) Pretreatment: Take 2g of spider silk, rinse it with purified water to remove impurities, and put it in an agate ball mill for crushing until the spider silk has a flocculent or powdery growth length of <1mm.

[0063] (2) Hydrolysis: Put the pulverized spider silk into a 1L glass container, add 200mL of 15g / L sodium hydroxide solution, and hydrolyze for 4.5h at a temperature of 85°C.

[0064] (3) Centrifugation: Centrifuge the hydrolyzate at a rotational speed of 15000r / min for 20min.

[0065] (4) Collection: Collect the centrifuged hydrolyzate into a glass container.

[0066] (5) Hydrolysis: hydrolyze the unhydrolyzed spider silk again, the method is the same as (2).

[0067] (6) Neutralization: neutralize with 1mol / L hydrochloric acid solution to pH 5.0.

[0068] (7) Filtration: filter the neutralized hydrolyzate throug...

Embodiment 3

[0070] Example 3 Preparation of spider silk hydrolyzate

[0071] The hydrolysis process of the present embodiment spider silk hydrolyzate is:

[0072] (1) Pretreatment: Take 2g of spider silk, rinse it with purified water to remove impurities, and put it in an agate ball mill for crushing until the spider silk has a flocculent or powdery growth length of <1mm.

[0073] (2) Hydrolysis: Put the pulverized spider silk into a 1L glass container, add 200mL of 25g / L sodium hydroxide solution, and hydrolyze for 4 hours at a temperature of 90°C.

[0074] (3) Centrifugation: Centrifuge the hydrolyzate at a rotational speed of 15000r / min for 20min.

[0075] (4) Collection: Collect the centrifuged hydrolyzate into a glass container.

[0076] (5) Hydrolysis: hydrolyze the unhydrolyzed spider silk again, the method is the same as (2).

[0077] (6) Neutralization: neutralize with 1mol / L hydrochloric acid solution to pH 5.5.

[0078] (7) Filtration: filter the neutralized hydrolyzate thr...

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Abstract

The invention relates to the technical field of cosmetics, and especially relates to a cosmetic containing spider silk hydrolysate. The cosmetic comprises the spider silk hydrolysate, and the spider silk hydrolysate is prepared by the following steps: spider silk is crushed, crushed spider silk and a sodium hydroxide solution are mixed, hydrolysis is carried out at 80-90 DEG C for 4-5 hours, centrifugation is carried out, and hydrolysate is obtained; pH value of the hydrolysate is adjusted to 4.0-5.5, the hydrolysate is filtered with a 200nm membrane, and the spider silk hydrolysate is obtained. The hydrolysis yield of amino acids is higher than 70%, and the amount of micro-molecule polypeptides is 72% or above; in the hydrolysate, methionine is not detected, except phenylalanine and tyrosine, yield of other amino acids is higher than 60%. The spider silk hydrolysate is used for masks for beauty treatment and skin care, beauty treatment characteristics of spider silk for water supplement, moisture retention, tightness, crease resistance, inflammation resistance, repair and the like are fully performed, the product characteristics are distinct, efficacy is substantial, and beauty treatment demands of people are satisfied.

Description

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Claims

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Application Information

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Owner HAINAN ZHUWANG PHARMA
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