Detection method for polychlorinated biphenyl of offshore fish

A technology for polychlorinated biphenyls and detection methods, which is applied to measurement devices, instruments, scientific instruments, etc., can solve problems such as unfavorable popularization and utilization, and difficulty in visual display of detection end points, and achieves the effect of convenient and fast solution and less interference peaks.

Active Publication Date: 2017-06-13
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the detection results of this method rely on the naked eye, and the detection end point is difficult to display intuitively, which is not conducive to popularization and utilization.

Method used

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  • Detection method for polychlorinated biphenyl of offshore fish
  • Detection method for polychlorinated biphenyl of offshore fish

Examples

Experimental program
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Embodiment 1

[0024] This protocol is a PCB kit.

[0025] A method for detecting polychlorinated biphenyls in offshore fish, including a pretreatment step and a detection step of a fish sample, the pretreatment step is to place the fish sample in a carbon dioxide extraction tank for supercritical extraction of carbon dioxide, and the extraction conditions are: extraction pressure 30.0~70.0MPa, extraction temperature 60~150℃, a mixture of acetone and methanol as a carrier, the content of the mixture in carbon dioxide is 3~5%, the flow rate of carbon dioxide is 25L / H, and the extraction time is 15-20 minutes, wherein the substance ratio of acetone and methanol is 1:2-5, and the fish meat extract is obtained.

[0026] The detection step uses a polychlorinated biphenyl kit, wherein the detection reagent includes the following components by weight:

[0027] 40 parts of acetone,

[0028] 4 parts of 37wt% concentrated hydrochloric acid,

[0029] 5 parts of disodium edetate,

[0030] Sodium nit...

Embodiment 2

[0037] The difference with embodiment 1 is:

[0038] The detection step uses a polychlorinated biphenyl kit, wherein the detection reagent includes the following components by weight:

[0039] 45 parts of acetone,

[0040] 6 parts of 37wt% concentrated hydrochloric acid,

[0041] 10 parts of disodium edetate,

[0042] 20 parts of sodium nitrite,

[0043] 8 parts of silver acetate,

[0044] 10 parts of dibutyl phthalate,

[0045] 15 parts of 2-ethyl-3,6-dimethylpyrazine,

[0046] 20 parts of phenanthroline,

[0047] 20 parts of anhydrous ether.

Embodiment 3

[0049] The detection step uses a polychlorinated biphenyl kit, wherein the detection reagent includes the following components by weight:

[0050] 42 parts of acetone,

[0051] 5 parts of 37wt% concentrated hydrochloric acid,

[0052] 6 parts of disodium edetate,

[0053] Sodium nitrite 13 parts,

[0054] 5 parts of silver acetate,

[0055] 7 parts of dibutyl phthalate,

[0056] 12 parts of 2-ethyl-3,6-dimethylpyrazine,

[0057] 9 parts of phenanthroline,

[0058] 13 parts of anhydrous ether.

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Abstract

The invention relates to a detection method for polychlorinated biphenyl of offshore fish. The method comprises a preprocessing step and a detecting step for a fish sample. The method is characterized in that the preprocessing step means placing the fish sample into a carbon dioxide extraction tank for performing carbon dioxide supercritical extraction; the extraction condition is as follows: the extracting pressure is 30.0-70.0MPa and the extracting temperature is at 60-150 DEG C; a mixed solution of acetone and methyl alcohol is taken as a carrying agent; the percentage of the mixed solution matter in carbon dioxide is 3%-5%; the carbon dioxide flow is 25L / H; the extraction time is 15-20 minutes; the mass ratio of acetone to methyl alcohol is 1:(2-5), thereby acquiring a fish extracting solution. The ultrasonic extraction is changed into carbon dioxide supercritical extraction, so that the dosage of the toxic dissolvent is greatly reduced, the enrichment ratio of polychlorinated biphenyl is increased and the detection efficiency is increased.

Description

technical field [0001] The invention relates to a method for detecting polychlorinated biphenyls in offshore fish. Background technique [0002] Polychlorinated biphenyls (PCBs) are one of the items that must be inspected for the export of aquatic products. They are important organic pollutants and last for a long time. The composition of congeners is relatively complex, and there are about 200 kinds. The current common standard analysis method is mainly the analysis of the total amount, and the detection of monomers is relatively difficult, and the detection of PCBs in aquatic food, especially in offshore fish, is even more difficult. The reason is that There are many kinds of offshore fish, and the pretreatment process of each kind of fish is different, which makes the detection difficult. [0003] Chinese patent documents CN102928539A The invention relates to an eluting device for sample preparation for detection of trace amounts of polychlorinated biphenyls in water by...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 罗红宇张飞何松泉
Owner ZHEJIANG OCEAN UNIV
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