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Method of industrially cultivating protocorm of dendrobium officinale without hormones

A technology of Dendrobium officinale and protocorm, which is applied in horticultural methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of large protocorm particles, decreased dryness rate, easy differentiation and germination, etc., and achieves improved dryness. the effect of increasing the multiplication rate

Active Publication Date: 2017-04-26
高蕴珺
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

General laboratories use pure solid medium to prepare protocorms, the dry rate is only 3~5%, and it is easy to differentiate and germinate during the preparation process, which will affect the subculture and proliferation
[0005] If pure liquid medium is used to prepare protocorms, the dry rate will be slightly higher than that prepared by solid culture by 2~3%, but in several subcultures, the proliferation speed of protocorms will decrease generation by generation, and the protocorms produced Particles are too large, easy to inactivate in the middle, and the drying rate gradually decreases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for cultivating protocorms of dendrobium officinale in a hormone-free industrialized manner, the steps are as follows:

[0023] (1) Sowing of Dendrobium officinale: Use the mature fruit pods of Dendrobium officinale as the induction raw material of protocorm, sow the seeds of Dendrobium officinale on the hormone-free medium G0, spread the seeds on the medium plane, the medium is about 200mL, the height is 2cm, and the seeds Spread a thin layer; the culture conditions are temperature 20°C, light 3000Lx, and culture for 12 days to obtain expanded Dendrobium officinale seeds;

[0024] (2) Protocorm induction: inoculate the expanded Dendrobium officinale seeds obtained in step (1) into liquid medium G1, the inoculation amount is 8 grams per 200 mL; place on a shaker and vibrate evenly at a frequency of 80 rpm, Keep the temperature at 20°C, light at 2000Lx, culture for 15 days and subculture once, after subculture twice, the seeds develop into protocorms;

[0025] ...

Embodiment 2

[0036] A method for cultivating protocorms of dendrobium officinale in a hormone-free industrialized manner, the steps are as follows:

[0037] (1) Sowing of Dendrobium officinale: Use the mature fruit pods of Dendrobium officinale as the induction raw material of protocorm, sow the seeds of Dendrobium officinale on the hormone-free medium G0, spread the seeds on the medium plane, the medium is about 200mL, the height is 2cm, and the seeds Spread a thin layer; the culture conditions are temperature 28°C, light 3000Lx, and culture for 15 days to obtain expanded Dendrobium officinale seeds;

[0038] (2) Protocorm induction: Inoculate the expanded Dendrobium officinale seeds obtained in step (1) into liquid medium G1, the inoculation amount is 10 grams per 200 mL; place on a shaker and vibrate evenly at a frequency of 110 rpm, Keep the temperature at 28°C, light at 3000Lx, and subculture once every 15 days. After subculture 3 times, the seeds develop into protocorms;

[0039] (3...

Embodiment 3

[0050] A method for cultivating protocorms of dendrobium officinale in a hormone-free industrialized manner, the steps are as follows:

[0051] (1) Sowing of Dendrobium officinale: Use the mature fruit pods of Dendrobium officinale as the induction raw material of protocorm, sow the seeds of Dendrobium officinale on the hormone-free medium G0, spread the seeds on the medium plane, the medium is about 200mL, the height is 2cm, and the seeds Spread a thin layer; the culture conditions are temperature 24°C, light 2500Lx, and culture for 14 days to obtain expanded Dendrobium officinale seeds;

[0052] (2) Protocorm induction: Inoculate the expanded Dendrobium officinale seeds obtained in step (1) into liquid medium G1, the inoculum amount is 9 grams per 200 mL; place on a shaker and vibrate evenly at a frequency of 100 rpm, Keep the temperature at 25°C and light at 2500Lx, culture for 15 days and subculture once, after subculture twice, the seeds develop into protocorms;

[0053]...

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PUM

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Abstract

The invention relates to a method of industrially cultivating protocorm of dendrobium officinale without hormones and belongs to the technical field of hormone-free cultivation. The hormone-free industrially cultivated dendrobium officinale is obtained by steps of sowing of dendrobium officinale, induction of protocorm, proliferation of protocorm and output of protocorm. The proliferation multiplying power of the protocorm is greatly improved and the stem yield thereof is improved, so that industrial production of the protocorm of dendrobium officinale has actual application value. As a hormone-free production mode is adopted, the obtained product meets related inspection requirements and can satisfy the demand in the market.

Description

technical field [0001] The invention relates to a hormone-free industrial cultivation method for dendrobium officinale protocorms, which belongs to the technical field of hormone-free cultivation. Background technique [0002] Dendrobium officinale (scientific name: Dendrobium officinale Kimura et Migo): stems erect, cylindrical, 9-35 cm long, 2-4 mm thick, unbranched, multi-noded; leaves bicolumn, papery, oblong-lanceolate, often with margins and middle ribs lavender. Racemes often emanate from the upper part of old stems that have fallen leaves, with 2-3 flowers; bracts dry membranous, light white, ovate, 5-7 mm long, sepals and petals yellow-green, nearly similar, oblong lanceolate, lip white, with a green or yellow callus at the base, ovate-lanceolate, slightly shorter than sepals, reflexed in the middle. Stamens yellow-green, about 3mm long, with 1 purple spot on both sides of apex; medicine cap white, long ovate-triangular, about 2.3mm long, apex subacute and 2-lob...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 高蕴珺
Owner 高蕴珺
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