A kind of candida krusei fluorescent PCR detection kit

Abstract

The invention relates to a candida krusei fluorescence PCR detection kit. The candida krusei fluorescence PCR detection kit comprises CD-PCR reactant liquor, the CD-PCR reactant liquor comprises a primer and a fluorescent probe A, the primer is divided into a primer F and a primer R, the nucleotide sequence of the primer F is as shown in SEQ ID No.1, the nucleotide sequence of the primer R is as shown in SEQ ID No.2, and the nucleotide sequence of the fluorescent probe A is as shown in SEQ ID No.3. The invention further relates to a method for detecting candida krusei. The kit has the advantages of high capacity of resisting disturbance, high sensitiveness, high specificity, high detection speed, convenience in operation and the like.

Description

technical field [0001] The invention relates to the field of detection of Candida, in particular to a fluorescent PCR detection kit for Candida krusei and a detection method thereof. Background technique [0002] Candida krusei (Candida krusei) belongs to the Saccharomycetes subphylum Bacillus class, Saccharomyces order, Saccharomyces family Candida genus. Candida species is a typical opportunistic fungus and the first pathogenic bacteria of invasive fungal disease (IFD). Candidiasis can involve human skin, mucous membranes and various internal organs. Invasive candidiasis is even more life-threatening, and candida bloodstream infection (BSI) is the fourth most common BSI in hospitals, and its crude case fatality rate can be as high as 39.2% (ICU 47.1%). Early diagnosis of candidiasis, especially invasive candidiasis, is often difficult, which can easily lead to delay in antifungal treatment and affect the prognosis of patients. [0003] In addition, Candida species can a...

AI Technical Summary

Problems solved by technology

Its advantages are simple, fast and cheap, but it can only distinguish Candida albicans from other cocci, and cannot distinguish Candida krusei from Candida albicans

[0009] 2. Culture method: It is an important basis for clinically determining the diagnosis and treatment of Candida at present, but clinically different Candida grows on Sabouraud medium and all present white colonies, so it is necessary to distinguish Candida krusei from Candida albicans Generally, CHROMagar medium is used for culture, which contains special chromogenic substances. When there are many pathogenic bacteria, the color of the colony is difficult to distinguish, which may easily lead to missed diagnosis.

This method currently plays a dominant role in the monitoring, prevention and control of daily nosocomial infection, but it has many shortcomings such as low resolution, many influencing factors, and poor repeatability.

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