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Application of oridonin combined with cryptotanshinone in the preparation of drugs for treating leukemia

A technology of oridonin and cryptotanshinone, applied in the field of biomedicine, can solve the problems of inhibiting proliferation, strong side effects, and poor targeting of chemotherapy regimens, and achieve the effect of wide application prospects

Active Publication Date: 2020-01-14
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects and deficiencies of existing drugs for treating leukemia, mainly due to the poor targeting of chemotherapy regimens (that is, no distinction between normal cells and cancerous cells, and mandatory blocking of the proliferation of all cells in the body), strong side effects , high cost and other disadvantages, to provide a combination of two active ingredients of Chinese herbal medicines with different anti-cancer mechanisms - Oridonin (Ori for short) and Cryptotanshinone (CPT for short) Compatibility method, both The significant synergistic effect between the two compounds can be used to achieve the goal of killing leukemia cells with low dose and high efficiency. At the same time, due to the low toxicity of Chinese herbal medicines, abundant sources and easy access to materials, it can greatly reduce the toxicity of drugs to the body during treatment. side effects and significantly lower treatment costs

Method used

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  • Application of oridonin combined with cryptotanshinone in the preparation of drugs for treating leukemia
  • Application of oridonin combined with cryptotanshinone in the preparation of drugs for treating leukemia

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Example 1 Study on Combination of Oridonin A and Cryptotanshinone in Chronic Myelogenous Leukemia Cell Lines

[0051] A certain concentration gradient of oridonin and cryptotanshinone were used to treat chronic myeloid leukemia K562 cells for 24 hours, while the concentration of the anticancer drug oridonin was fixed, and cryptotanshinone with different gradient concentrations was used to treat leukemia K562 cells 24 Hours, the MTT colorimetric method was used to calculate the inhibitory intensity of cell proliferation under three different drug treatment modes.

[0052] The specific research methods are as follows:

[0053] 1. Experimental cell lines

[0054] K562 chronic myelogenous leukemia cells were obtained from the Bioengineering Research Center of Sun Yat-sen University (Guangdong, Guangzhou).

[0055] 2. Cell culture:

[0056] K562 cells were cultured with complete medium (RPMI-1640 medium containing 10% FBS and 1% double antibody) for routine cell culture a...

Embodiment 2

[0075] Example 2 Study on Combination of Oridonin A and Cryptotanshinone in Acute Myeloid Leukemia Cell Lines

[0076] A certain concentration gradient of oridonin and cryptotanshinone were used to treat acute myeloid leukemia HL60 cells for 24 hours, while the concentration of the anticancer drug oridonin was fixed, and cryptotanshinone with different gradient concentrations was used to treat leukemia HL60 cells 24 Hours, the MTT colorimetric method was used to calculate the inhibitory intensity of cell proliferation under three different drug treatment modes.

[0077] The specific research methods are as follows:

[0078] 1. Experimental cell lines

[0079] HL60 acute myeloid leukemia cells were obtained from the Bioengineering Research Center of Sun Yat-sen University (Guangdong, Guangzhou).

[0080] 2. Cell culture:

[0081] HL60 cells were cultured with complete medium (RPMI-1640 medium containing 10% FBS and 1% double antibody) for routine cell culture at a constant t...

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Abstract

The invention discloses applications of mixed oridonin and cryptotanshinone in preparing a medicine for treating leukemia. The research finds that after the traditional Chinese medicinal active ingredients with different anti-cancer mechanisms, namely, oridonin and cryptotanshinone are mixed at a suitable ratio, a significant synergistic interaction effect can be generated by the mixture. Through the mixing method, a medicinal composition with low concentration can kill many leukemia cells, meanwhile, the total medicine concentration available when the inhibition ratio of the medicine for the leukemia cell proliferation achieves 50% can be effectively reduced, and moreover, in the mixing method, the use amounts of the traditional Chinese medicinal active ingredients are not within the range capable of causing harm to the body. Therefore, the oridonin and cryptotanshinone mixing scheme has great significance and wide application prospects in the aspect of preparing the medicine for treating leukemia and the aspect of prevention and treatment as well as study on leukemia.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the application of oridonin combined with cryptotanshinone in the preparation of drugs for treating leukemia. Background technique [0002] Leukemia, commonly known as "blood cancer", is a type of malignant blood tumor disease derived from hematopoietic stem cells. Currently, the treatment of leukemia mainly focuses on the following regimens: chemotherapy, radiotherapy, bone marrow transplantation, and immunotherapy, among others. Among them, chemotherapy has become the mainstream scheme in the treatment of leukemia because of its relatively strong targeting and relatively low treatment cost. [0003] In the commonly used chemotherapy regimens, the idea of ​​drug combination has been fully reflected. At present, most of the combination medicines commonly used in clinical practice are Western medicine combined with Western medicine and Western medicine combi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/537A61K31/58A61P35/02A23L33/10A61K31/352
CPCA23V2002/00A61K31/352A61K31/58A61K36/537A61K45/06A61K2300/00A23V2200/308
Inventor 董博文周惠屈良鹄
Owner SUN YAT SEN UNIV
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