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Detection method for peripheral blood VEGF of renal cancer patient

A detection method and peripheral blood technology, applied in the field of VEGF detection, can solve the problems of unsuitable tissue sample collection, inability to evaluate the VEGF expression status of patients, etc., and achieve the effect of real-time detection

Inactive Publication Date: 2016-05-04
山东省医学科学院
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Problems solved by technology

[0005] In order to overcome the inadequacy of taking tissue samples from patients with advanced renal cancer and the inability to evaluate the expression status of VEGF in patients, the present invention provides a method for detecting VEGF in peripheral blood of patients with renal cancer: using a membrane filtration device to separate and obtain peripheral blood from patients with advanced renal cancer CTCs in cells; use cell wax block technology to make thin-layer sections, and then detect the expression of VEGF gene in patients with renal cancer

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  • Detection method for peripheral blood VEGF of renal cancer patient
  • Detection method for peripheral blood VEGF of renal cancer patient
  • Detection method for peripheral blood VEGF of renal cancer patient

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[0037] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0038] An example of using this technique to separate, obtain and identify VEGF gene expression in peripheral blood circulating tumor cells of 8 patients with advanced renal cancer (while detecting 8 normal samples as negative controls).

[0039] Collect 5ml of peripheral blood from each sample, dilute the peripheral blood with 45ml diluent (composition: 1mmol / lEDTA+0.1%BSA), and then add 3ml of 4% paraformaldehyde to fix the diluted blood sample for 10 minutes. At fixed intervals, assemble the membrane filtration device: as attached figure 1 , figure 2 , image 3 As shown, the filter device is composed of a filter 3 , a filter membrane 7 , a blood sample container 2 , a waste liquid tank 5 , and an iron stand 1 .

[0040] Use a membrane filtration device to separate peripheral blood samples and enrich circulating tumor cells in peripheral blood: We...

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Abstract

The invention discloses a detection method for peripheral blood VEGF of a renal cancer patient. The method comprises the following steps: separating CTC (circulating tumor cell) in the peripheral blood of the advanced renal cancer patient by utilizing a membrane filtering device; producing a thin cell layer by utilizing a cell paraffin block technology, and further detecting the genic expression condition of the VEGF of the renal cancer patient. By virtue of the technological method, the VEFG expression condition of the renal cancer patient can be detected without sampling the renal cancer tissue. The technique is minimally invasive and even noninvasive, and the detection can be performed in real time.

Description

technical field [0001] The invention relates to a new method for detecting VEGF, especially a method for detecting VEGF in renal cancer patients through peripheral blood. Background technique [0002] Hypoxia-induced signaling pathway plays an important role in the occurrence and development of RCC, and its signal changes can affect the downstream VEGF pathway, promote tumor angiogenesis, and cause tumor progression. Therefore, VEGF can not only be used as an indicator of prognosis, but also become a new target for tumor treatment. Studies have shown that VEGF can be used as an indicator of renal cancer stage, grade and prognosis, and provide a basis for targeted drug or cytokine adjuvant therapy after renal cancer surgery. Currently, targeted drugs targeting VEGF and its related receptors, such as Sola Fini, sunitinib malate, etc., have become the first-line treatment drugs for advanced renal cell carcinoma. Therefore, it is very important to correctly detect the expressi...

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/68G01N33/56966G01N33/57438
Inventor 李胜孙萍王振丹
Owner 山东省医学科学院
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