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Fusion peptide promoting osteoblast adhesion and osteogenic differentiation, preparation method and application

A technology of cell adhesion and osteogenic differentiation, applied in the fields of biology and medicine, can solve the problems of lack of osteogenic differentiation, adhesion, proliferation, etc., and achieve the effect of promoting adhesion, good application prospects, and proliferation promotion.

Active Publication Date: 2019-12-13
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the hydrogel structure can simulate the extracellular matrix (extracellular matrix, ECM) and its microenvironment, provide cells with a good in vitro growth environment, and is conducive to cell adhesion, proliferation and differentiation; Glue lacks the ability to promote osteogenic differentiation

Method used

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  • Fusion peptide promoting osteoblast adhesion and osteogenic differentiation, preparation method and application
  • Fusion peptide promoting osteoblast adhesion and osteogenic differentiation, preparation method and application
  • Fusion peptide promoting osteoblast adhesion and osteogenic differentiation, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Peptide design

[0028] Using RADA16-I peptide, the sequence is SEQ ID NO:1 (AcN-RADARADARADARADA-CONH 2 ) As a basis, a peptide bond is used to directly couple an osteostatin peptide (TRSAW) at the C-terminus. The amino acid sequence of the peptide (fusion peptide) is SEQID NO: 2 (RADARADARADARADAGGTRSAW) with a molecular weight of 2387.54; or in the C of the RADA16-I peptide The end is coupled to the osteostatin peptide (TRSAW) via two amino acid residues GG. The amino acid sequence of the peptide (fusion peptide) is SEQ ID NO: 3 (RADARADARADARADAGGWASRT). The above peptides are all synthesized by Suzhou Qiangyao Biotechnology Co., Ltd.

Embodiment 2

[0029] Example 2 Polypeptides can promote cell adhesion, proliferation and differentiation

[0030] 1. Preparation of self-assembled peptide mother liquor

[0031] Add 1ml of sterile ultrapure water to the peptide powder (each 10mg aliquot), pipette and mix well, and ultrasonically treat the solution with an ultrasonic cleaner at room temperature for 30min to remove the viscosity of the solution to obtain 1% (m / v) peptide mother liquor. Take RADA16-I and RADA16-TRSAW mother liquor and mix in equal volume to obtain 1% (m / v) TRSAWmx mother liquor.

[0032] 2. Atomic force microscope (AFM) observes the fiber structure of self-assembled peptides

[0033] Dilute the mother liquor of TRSAW, RADA16-I, RADA16-TRSAW and TRSAWmx with ultrapure water to obtain a dilution of 0.5‰ (m / v), and ultrasonic treatment with an ultrasonic cleaner at room temperature for 30 minutes. Take 5 μL of different peptide dilutions and add them to the clean round mica sheet. After standing for 15 seconds, rinse w...

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Abstract

The invention provides a fusion peptide capable of promoting osteoblast adhesion and osteogenic differentiation as well as a preparation method and application of the fusion peptide. The fusion peptide comprises an RADA16-I peptide and an osteostatin (TRSAW) peptide coupled at the C end of the RADA16-I peptide sequence, wherein the amino acid sequence of the RADA16-I peptide is shown as SEQ ID NO:1 in the specification. The invention further provides a medicine composition comprising the fusion peptide. The fusion peptide provided by the invention not only can promote MC3T3-E1 osteocyte adhesion and proliferation, but also can promote osteogenic differentiation, and further has a good application prospect.

Description

Technical field [0001] The present invention relates to the fields of biology and medicine. Specifically, the present invention relates to a novel polypeptide that can promote the adhesion and osteogenic differentiation of osteogenic-related cells and its application. Background technique [0002] Bone transplantation is a common clinical treatment method for repairing bone defects caused by spinal fusion, bone injury and bone tumors. Autologous bone transplantation has always been used as the gold standard for bone transplantation, but its clinical application is limited due to limited materials, pain in the operation area, and increased risk of bleeding and infection. Scientists have been trying to find a variety of alternative materials with osteoconductivity, osteogenic activity and osteoinductive properties. At present, a variety of synthetic bone substitute materials have been used clinically, such as hydroxyapatite, ceramic materials, etc., but synthetic bone substitute m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C07K1/00A61K38/16A61P19/08
CPCA61K38/00C07K14/00C07K2319/00
Inventor 侯天勇陈贝克邢军超许建中
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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