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Preparation method of zinc chelating peptide from shrimp leftovers

A technology of leftovers and chelated peptides, applied in animal protein processing, protein food ingredients, fermentation, etc., can solve the problems of high cost of protein and astaxanthin

Active Publication Date: 2020-10-13
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, my country is mainly used to produce chitin, and the recycling of protein and astaxanthin is industrialized due to the high cost.
[0003] After retrieval, there is no report on the preparation of zinc chelating peptides from shrimp processing waste

Method used

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  • Preparation method of zinc chelating peptide from shrimp leftovers
  • Preparation method of zinc chelating peptide from shrimp leftovers
  • Preparation method of zinc chelating peptide from shrimp leftovers

Examples

Experimental program
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Embodiment

[0021] 1) Preparation of protein enzymatic hydrolyzate from shrimp processing waste: crush the frozen shrimp processing waste (shrimp head and shrimp shell) with a tissue grinder, and add phosphate buffer (0.2 mol / L, pH 7.0), sonicated at 47°C for 90 min at 500W ultrasonic power, then added neutral protease (enzyme activity ≥ 5.0×10 4 U / g), after incubating at 47°C for 2 hours, the temperature was raised to 95°C and maintained for 10 minutes; the temperature of the solution was lowered to 37°C, the pH was adjusted to 7.8, and trypsin (enzyme activity ≥ 2.5×10 4 U / g) after heat preservation for 3 h, the temperature rose to 95°C and kept for 10 min, then at 10 000 g Centrifuge for 15 min to obtain the supernatant, which is the proteolyzate of shrimp processing waste.

[0022] 2) Preparation of zinc chelating peptide from shrimp processing waste: the prepared enzymatic hydrolyzate was subjected to ultrafiltration treatment with a 3 kDa ultrafiltration membrane, and the fracti...

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PUM

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Abstract

The invention relates to a method for preparing a zinc chelated peptide by utilizing shrimp meat offal. The preparation method particularly comprises the following steps: adopting the shrimp meat offal as a raw material, and extracting collagen according to an acid process; conducting enzymolysis on the collagen by utilizing pepsase and trypsin; carrying out ultrafiltration, macroporous resin column chromatography, gel column chromatography, and reversed-phase high performance liquid chromatograph separation and purification to obtain the zinc chelated collagen peptide. The zinc chelated collagen peptide has a unique chelated and operating mechanism, thereby being high in absorption and availability; meanwhile, the zinc chelated collagen peptide can be utilized for supplementing both polypeptides / amino acid and zinc, thereby being an ideal zinc-supplementing substance.

Description

technical field [0001] The invention belongs to the technical field of deep processing of aquatic products, and relates to a preparation method of an ion chelating peptide, in particular to a preparation method of a zinc chelating peptide from shrimp leftovers. Background technique [0002] my country is very rich in shrimp resources. In recent years, with the rapid development of the shrimp processing industry, a large number of processing wastes have been produced, which are rich in effective substances such as protein, chitin and astaxanthin. After investigation, it was found that when the production enterprises that use shrimp as the main raw material process shrimp processing waste, some of them are sold as waste at very low prices, or transported to farms for drying and crushing as feed. The leftovers were eventually transported directly to the garbage dump due to moldy and rotted piles. Not only cause serious waste of resources, but also cause environmental pollution...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06A23J3/04A23J3/34
Inventor 赵玉勤徐银峰王斌陈荫孙坤来
Owner ZHEJIANG OCEAN UNIV
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