Ovinetheileriasis immuno colloidal gold detection test paper strip and preparation method thereof

Abstract

The invention discloses an ovinetheileriasis immuno colloidal gold detection test paper strip for rapidly detecting theileria uilenbergi and theileria luwenshuni antibodies in sheep serum. The ovinetheileriasis immuno colloidal gold detection test paper strip comprises a PVC pad, wherein a sample pad for feeding of a sample to be detected, a colloidal gold pad coated with gold marked theileria uilenbergi TuIP recombinant protein antigen, a nitrocellulose membrane and a water absorbing pad are sequentially arranged on the upper surface of the PVC pad; one end of the sample pad is arranged above one end of the colloidal gold pad; the other end of the colloidal gold pad is arranged above one end of the nitrocellulose membrane; one end of the water absorbing pad is arranged above the other end of the nitrocellulose membrane; the nitrocellulose membrane is coated with a detection line of the theileria uilenbergi TuIP recombinant protein antigen and a quality control line of a polyclonal antibody. The invention further provides a preparation method of the detection test paper strip. The detection test paper strip prepared by using the preparation method has the characteristics of small sample amount and simplicity in operation and is simple, convenient and rapid in detection, low in cost and applicable to use for basic level veterinarian laboratories and veterinarian operators, no complex instrument is needed, and the result can be directly judged by naked eyes.

Description

technical field [0001] The invention belongs to the technical field of sheep theileriasis detection, and in particular relates to a sheep theileriasis immune colloidal gold detection test strip and a preparation method thereof. Background technique [0002] Theileria uilenbergi & T. luwenshuni belong to Protista, Apicomplexa, Sporozoea, Piroplasmia, Piroplasma The order (Piroplasmida), Theileriidae (Theileriidae), and Theileria (Theileria) are the pathogens that cause theileriosis in sheep. Blood tick Qinghai and blood tick longhorn are the vectors of the disease. Theileriasis of sheep is widely distributed in my country, and the pathogen or antibody has been detected in Sichuan, Qinghai, Gansu, Liaoning, Inner Mongolia, Shanxi, Ningxia, Xinjiang, Henan, Hubei and other places. The mixed infection of Theileria yoshii and Theileria rushii is relatively common, and the infection often causes characteristic clinical symptoms such as high fever, anemia, jaundice, dyspnea, and ...

AI Technical Summary

Problems solved by technology

These methods require corresponding instruments and equipment, are suitable for laboratory testing, and cannot be used for clinical examination at present

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