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A method for cultivating triploid Dendrobium Huoshanense by using the fusion breeding method of diploid cells and pollen cells

A technology of diploid cells and pollen cells is applied in the field of biological breeding of medicinal plants of the genus Dendrobium orchid, and can solve the problems that no public report of triploid Dendrobium Huoshan has been reported.

Active Publication Date: 2016-12-14
WEST ANHUI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, only the cultivation methods of triploid Dendrobium officinale and Dendrobium spring have been published and authorized. So far, there has been no public report on the triploid Dendrobium Huoshanense (chromosomal karyotype index is 2n=3x=57) and the cultivation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0023] (1) The plants of variety A and variety B are planted and cultivated according to conventional methods;

[0024] (2) The seeds of the above variety A are aseptically sown in a solid medium to obtain the callus of the protocorm. The solid medium is 1 / 2MS medium with halved macronutrients, and 0.2g / L 2,4 -D and 0.2g / L NAA, adjust the sucrose concentration to 5%-8%, add 10-15% coconut milk, culture temperature in the range of temperature difference between day and night at 25-18°C, light time 10-12h, light intensity 2000 -3000Lux, color temperature is 4500-5500K;

[0025] (3) Aseptically culture the pollen of the above variety B in a solid medium to obtain pollen cell populations, the solid medium is H medium, add 0.5g / L 2,4-D and 1.5g / L NAA, and cultivate The temperature is within the diurnal temperature range of 25-18°C, the light time is 10-12h, the light intensity is 2000-3000Lux, and the color temperature is 4500-5500K;

[0026] (4) Prepare the protoplasts of pollen...

Embodiment 2

[0030] (1) The plants of variety A and variety B are planted and cultivated according to conventional methods;

[0031] (2) The seeds of the above variety B are aseptically sown in a solid medium to obtain the callus of the protocorm. The solid medium is 1 / 2MS medium with halved macronutrients, and 0.2g / L 2,4 -D and 0.2g / L NAA, adjust the sucrose concentration to 5%-8%, add 10-15% potato juice, culture temperature in the range of temperature difference between day and night at 25-18°C, light time 10-12h, light intensity 2000 -3000Lux, color temperature is 4500-5500K;

[0032] (3) Aseptically culture the pollen of the above variety A in a solid medium to obtain pollen cell populations, the solid medium is H medium, add 0.5g / L 2,4-D and 1.5g / L NAA, and cultivate The temperature is within the diurnal temperature range of 25-18°C, the light time is 10-12h, the light intensity is 2000-3000Lux, and the color temperature is 4500-5500K;

[0033] (4) Prepare the protoplasts of pollen...

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Abstract

The invention discloses a method for cultivating triploid dendrobium huoshanense by a fusion breeding method of diploid cells and pollen cells. The method comprises the following steps: (1) selecting dendrobium huoshanense varieties from different producing areas, which are good in stress resistance, quick in growth, thick in stalks, long in stem height, various in tillering, low in fiber content and high in medicinal composition content as a triploid cultivating source plant parent; (2) performing aseptic seeding on source plant seeds in a solid culture medium to obtain protocorm; (3) performing sterile culture on the source plant pollen in the solid culture medium to obtain a pollen cell population; (4) preparing protoplasts of the pollen cells and the protocorm cells to perform cell fusion; (5) culturing the fused cells on the solid culture medium to differentiate the fused cells into young plants, and then detecting the triploid plants; and (6) performing tissue culture on the triploid plants to obtain a large number of triploid young plants.

Description

technical field [0001] The invention relates to a biological breeding method of medicinal plants of the genus Dendrobium orchidaceae, in particular to a method for cultivating triploid Dendrobium Huoshanense by using the fusion breeding method of diploid cells and pollen cells. Background technique [0002] Dendrobium huoshanense (scientific name: Dendrobium huoshanense C.Z.Tang et S.J.Cheng) has a history of more than 200 years. Native to my country's Anhui Dabie Mountains and other places, it is the first protected variety among more than 200 endangered and scarce varieties in the national major modern Chinese medicine project. It is the best product among medicinal Dendrobium, and it is also a rare and precious Chinese herbal medicine unique to Huoshan County with geographical indications of origin. Both "Shen Nong's Materia Medica" and "Compendium of Materia Medica" have records. The polysaccharides and alkaloids contained in Dendrobium Huoshanense have the effects of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 邓辉姚厚军张德华杨国栋陈乃东
Owner WEST ANHUI UNIV
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