A kind of breeding method of radish maintainer line with ogura male sterile cytoplasm

A male sterile line, male sterile technology, applied in the direction of botany equipment and methods, biochemical equipment and methods, microbial determination/inspection, etc.

Inactive Publication Date: 2015-12-30
SHANDONG CROP GERMPLASM CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] When using the Ogura male sterile line for crop breeding, scientists often choose to select the maintainer line from plants without Ogura male sterile cytoplasm. From the current literature and related materials, the cytoplasm of the maintainer line of the Ogura male sterile line There are only two types, one is the normal cytoplasmic type (N type) and the other is the NWB cytoplasmic type, and no one has bred maintainer lines from plants with Ogura cytoplasm

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  • A kind of breeding method of radish maintainer line with ogura male sterile cytoplasm
  • A kind of breeding method of radish maintainer line with ogura male sterile cytoplasm
  • A kind of breeding method of radish maintainer line with ogura male sterile cytoplasm

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Embodiment Construction

[0035] The present invention will be described in detail below in conjunction with the accompanying drawings and implementation examples.

[0036] First select the male sterile material and the proposed maintainer line material, the male sterile material selected in the present invention is RBY imported from abroad, and RBY is the type of Japanese green radish; the proposed maintainer line material is Shandong local variety Zaozhuang Dahongpao, Zaozhuang Dahong The gown is red-skinned and white-hearted radish. After selecting the material, carry out the PCR molecular marker and fragment sequencing of the cytoplasmic type. The sterile line is required to have OguraH-type cytoplasm, and the maintainer line has OguraA-type cytoplasm.

[0037] Ogura-specific primers: ORF-F5'-TTCAAATCCTGTCCCCGCACC-3' and ORF-R5'-GCCTTACACCATTGGGATACTTC-3' were used to carry out PCR molecular markers on the sterile material RBY and the proposed maintainer line material Zaozhuang Dahongpao. In order...

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Abstract

The invention belongs to the technical field of crop breeding, and in particular discloses a radish maintainer line with Ogura male-sterile cytoplasms and a breeding method for the radish maintainer line with the Ogura male-sterile cytoplasms. The radish maintainer line with the Ogura male-sterile cytoplasms is characterized by comprising the Ogura male-sterile cytoplasms, wherein the maintainer line has a maintaining function on a sterile line with Ogura H-shaped male-sterile cytoplasms; female and male organs of the maintainer line normally grow and are high in self-fruitfulness; pistils of a male sterile line normally grow, and stamens pistils of the male sterile line are completely degraded and do not have effective pollens. The radish maintainer line breaks through the breeding range and the breeding method of conventional maintainer lines; the maintainer line which has the maintaining function on an Ogura cytoplasms male sterile line is selected from fertile plants with an Ogura cytoplasm type, so that the breeding range of the maintainer line is further expanded, and the very important significance on further research on the male sterility of the Ogura cytoplasms is achieved.

Description

(1) Technical field [0001] The invention belongs to the technical field of crop breeding, in particular to a breeding method for a radish maintainer line with Ogura male sterile cytoplasm. (2) Background technology [0002] There is obvious heterosis in cruciferous plants. Because of their small flower organs, manual detasseling operation is time-consuming and laborious, and there are few hybrid seeds obtained by one pollination. To solve this problem, breeders choose self-incompatibility. But the self-incompatibility line is easily affected by factors such as the environment, the compatibility is unstable, and the purity of the seed production is often not high. The use of plant male sterile lines for hybrid seed production can better solve the problem of self-incompatible line seed production, and can improve the purity of hybrid seeds and increase the yield of crops. It has become the main development direction of the international seed industry. [0003] The male steril...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H5/06A01H1/02A01H1/04C12Q1/68
Inventor 王效睦王俊峰白静丁汉凤孔维国李娜娜宫永超
Owner SHANDONG CROP GERMPLASM CENT
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