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The technology of total artificial seedling cultivation of Hemerocallis

A kind of technology of japonica and artificial, which is applied in the technical field of full artificial seedling raising of japonica, which can solve the problems of poor attachment and low density of seedlings, and achieve the effects of simple operation, reduced production cost and enhanced reliability

Inactive Publication Date: 2015-10-07
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defects and drawbacks of the prior art, the purpose of the present invention is to provide a technique for the artificial cultivation of Hemerocallis using spores, which can not only improve the utilization rate of germplasm, but also solve the problems of poor attachment and low density of seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In late February of that year, Xuanzana was collected from the waters near Long Island in Shandong. The algae body chose a mature leaf -shaped body with dark brown body, the surface with a pork spouse, and the hollow festival, and washed the surface and outside the surface of the tube -shaped algae in the sterilized seawater.After the washed algae is placed in a 12 ° C training box for 3 hours, it was put into a pre -cloth disinfection seawater in the predetermined with the female and male.The slice of algae is cultivated in a 250ml triangular bottle. The cultivation conditions are 16 ° C in water temperature, 12 hours long, and the light strength is 18.0-54.0 μmol / (M M M M. 2 · S), the water body is replaced every 3-5 days.After 40 days of cultivation, Hezi developed into a silk body, which is the germplasm of the year.

[0024] The quality of the use of Xuanzao began to expand on June 10, the amplification conditions were temperature 21 ° C, the light period was 14 hours,...

Embodiment 2

[0027]The method of obtaining the germplasm of the Xingjie is the same as the implementation example 1. For many years, it has collected Xingjie Genomes in different regions to establish the Xingjie Genomic Library.The selection of healthy germs from this mass library began to expand on June 1st, and the expansion was performed by inflatable training. Other conditions were temperature 20 ° C, 12 hours of light, and the light strength of 72.0 μmol / (M M M. 2 · S).On September 26th, the germplasm after the amplification, the condition is 18 ° C, the light time is 10 hours, and the light intensity is 21.6 μmol / (m M 2 · S).Control the nutritional salt NO in the water body 3 — N (10-40mg / ml), PO 4 3— P (20-80 mg / ml), the water body is replaced every 5-7 days.From 26 days to October 21, Xingzao single-chamber spores are mature, characterized by: color becomes dark brown, cells swell significantly, with a diameter of 60-80 μm.The ripe quality of the mature spores were performed on Octob...

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Abstract

The invention relates to a total artificial scytosiphon lomentaria breeding technology. The total artificial scytosiphon lomentaria breeding technology comprises the following steps: (1) performing germplasm preparation, namely preparing germplasm from scytosiphon lomentaria collected in the current year, or preparing the germplasm by the recovery of the germplasm stored in the previous years; (2) performing germplasm amplification, namely amplifying the germplasm of the scytosiphon lomentaria by using an airlift photo-bioreactor; (3) performing germplasm induction, namely inducing the germplasm of the scytosiphon lomentaria to generate a mature unilocular sporangium; (4) performing spore seedling collection, namely stimulating the unilocular sporangium to diffuse spores, and collecting the spores on a net curtain formed by blending vinylon and polyvinyl chloride; (5) performing indoor seedling breeding; (6) performing mariculture, namely performing mariculture in a raft culture manner for 60-80 days to breed commercial scytosiphon lomentaria of which the body length is 60-100cm, wherein 80-120 seedlings exist on a seedling rope which is 1cm long. According to the total artificial scytosiphon lomentaria breeding technology, the scytosiphon lomentaria breeding efficiency can be improved, and the stable yield of commercial seedlings is ensured. The technology has the advantages of high germplasm utilization rate, firm seedling adhesion, low breeding cost, good production benefit and the like, and can be widely applied to large-scale industrial breeding and artificial culture of the scytosiphon lomentaria.

Description

Technical field [0001] The present invention involves a seaweed breeding technology, which specializes in a Xingjie whole artificial seedling technology. Background technique [0002] Xuanzao (Xuanzao ( Scytosiphon Lomentaria ) It belongs to the brown algae door ( Phaeophyta ), The warm seaweed, is widely distributed in my country in the north from the north to the south of Liaonong Peninsula to the coastal areas between Hailing Island, Guangdong Province.Xuanzao has a history of alien generation alternation. The macro tubular algae body is the phase of the supporting body, and the micro -shaped, mat, and cushion -shaped sides are the stage of spores.Satellite and cushion forms to form silk.Xuanzani silk -shaped body has the characteristics of easy preservation and rapid growth. It is a material for Xie algae to keep, amplification, and seedlings. [0003] Xingjang is rich in brown alginic acid, protein, insoluble dietary fiber, EPA, and also has nutritional ingredients such as a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00
CPCY02A40/80
Inventor 宫相忠高伟张文健邢永泽张必达
Owner OCEAN UNIV OF CHINA
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