Preparation method of freeze-dried rabbit brain tissue factor

Abstract

The invention discloses a preparation method for a rabbit brain tissue factor freeze-drying preparation. The preparation method comprises the following steps: taking a fresh rabbit brain and stripping meninx and blood vessels; removing a brainstem and washing by using normal saline; putting the rabbit brain into a cleaning mortar and adding acetone until the rabbit brain is immersed; grinding by using a grinding hammer and pouring off the acetone; repeating for three times; filtering the acetone by using filtering paper; putting the rabbit brain into an oven of 37 DEG C and paving to evaporate and dry the acetone; adding the normal saline; uniformly agitating and putting a mixture into a constant-temperature water bath of 37 DEG C to be pre-heated and activated, incubated and agitated; centrifuging for separating; taking liquid supernatant and filtering to obtain a rabbit brain FIII tissue factor suspension solution; adding the rabbit brain FIII tissue factor suspension solution into a small bottle; freezing-drying, exhausting and pressing a bottle to prepare the rabbit brain tissue factor freeze-drying preparation, and storing at -20 DEG C. With the adoption of the scheme, the technology is very mature, the quality is very stable and the guarantee period is very long; the preparation method is very convenient to use; the preparation method has very high medical teaching, scientific research, clinical diagnosis and treatment and economic values.

Description

technical field [0001] The invention belongs to the technical field of biomedical preparations. More specifically, the present invention relates to a preparation method of freeze-dried rabbit brain tissue factor. Background technique [0002] In the 1970s, in order to study disseminated intravascular coagulation (DIC), the rabbit (dog) brain powder (FⅢ: crude product of tissue factor) was developed. Rabbit (dog) brain powder (FⅢ: crude product of tissue factor) is used as the initiating factor of disseminated intravascular coagulation (DIC) animal model and the hematological diagnostic indicators of DIC prothrombin time (PT) and activated partial thromboplastin time (APTT) ) detection is of great value. [0003] However, in the prior art, rabbit brain thromboplastin (FⅢ: tissue factor) has only powder, and only a prothrombin time (PT) assay kit made with rabbit brain thromboplastin. When in use, it needs to be preheated and activated for an hour, and then centrifuged, fil...

AI Technical Summary

Problems solved by technology

[0004] In addition, the shelf life of the preparation in the prior art is shorter, only 8 months

[0005] However, in the prior art, only rabbit brain powder is commercially available (actually only FⅢ: crude product of tissue factor)

When in use, it needs to be activated by preheating, incubated for 60 minutes, and then centrifuged, filtered, etc. before use, which is troublesome to use

In addition, the technical quality of rabbit brain powder in the prior art is unstable, and the shelf life is short, and the shelf life of -20C storage is only 6 months