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Bacillusamyloliquefaciens and validamycin compound biological bactericide and application thereof

A biofungicide and amylolytic spore-resolving technology, applied in the directions of fungicides, biocides, applications, etc., can solve the problems of excessive pesticide residues, affecting the quality of high-quality rice, and polluting the ecological environment of farmland, so as to reduce the cost of drug use and reduce drug use. The number of times, the effect of expanding the scope of prevention and treatment

Inactive Publication Date: 2014-04-30
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to propose an important disease to rice——bacterial blight, aiming at the current problems of using chemical pesticides in large quantities to prevent and control rice diseases, seriously polluting farmland ecological environment, causing pesticide residues to exceed the standard, and affecting the quality of high-quality rice. Bacillus amyloliquefaciens Jinggangmycin compound biofungicide with good control effect on sheath blight and its application in controlling rice diseases

Method used

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  • Bacillusamyloliquefaciens and validamycin compound biological bactericide and application thereof
  • Bacillusamyloliquefaciens and validamycin compound biological bactericide and application thereof
  • Bacillusamyloliquefaciens and validamycin compound biological bactericide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、2

[0019] Embodiment 1, the preparation of 2.5% Jinggangmycin · 3 billion live spores / milliliter bacillus amyloliquefaciens water

[0020] Effective drugs:

[0021] 5% Jinggangmycin aqueous solution was produced and provided by Tonglu Huifeng Biotechnology Co., Ltd., Zhejiang Province.

[0022] 6 billion live spores of Bacillus amyloliquefaciens Lx-11 / ml water solution (hereinafter referred to as 6 billion live spores / ml water solution) is produced and provided by Jiangsu Suke Agrochemical Co., Ltd.

[0023] Additive: NNO (sodium methylene bis-naphthalene sulfonate), outsourced.

[0024] 5% Jinggangmycin water preparation and 6 billion live spores / ml water preparation are prepared according to weight percentage 1:1, and 1% of auxiliary agent NNO gross weight is prepared, namely:

[0025] 5% Jinggangmycin aqueous solution: 49.5%; 6 billion live spores / ml aqueous solution: 49.5%; additive NNO: 1%.

[0026] Preparation process: put 5% Jinggangmycin water agent and 6 billion live ...

Embodiment 2、2

[0027] Example 2, Determination of synergistic effect of 2.5% Jinggangmycin 3 billion live spores / milliliter Bacillus amyloliquefaciens water agent on growth of bacterial blight of rice

[0028] The tested strains: rice bacterial blight: XooZ173 was provided by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences.

[0029] Tested medicament: 2.5% Jinggangmycin·3 billion live spores / ml aqueous solution of Bacillus amyloliquefaciens provided by Example 1.

[0030] Contrasting drugs:

[0031] 5% Jinggangmycin aqueous solution was produced and provided by Tonglu Huifeng Biotechnology Co., Ltd., Zhejiang Province.

[0032] The 6 billion live Bacillus amyloliquefaciens spores / ml water solution is produced and provided by Jiangsu Suke Agrochemical Co., Ltd.

[0033] Indoor synergistic bioassay:

[0034] XooZ173 strain of bacterial blight of rice to be tested was activated on NA (contains 3.0g of beef extract, 5.0g of peptone, 2.5g of glucose, and 18.0g of ag...

Embodiment 3、2

[0047] Example 3, Determination of synergistic effect of 2.5% Jinggangmycin 3 billion live spores / milliliter Bacillus amyloliquefaciens water agent on rice sheath blight mycelia growth

[0048] Tested strains:

[0049] Rice sheath blight RH-2 was provided by the Plant Protection Institute of Jiangsu Academy of Agricultural Sciences.

[0050] Medication tested:

[0051] 2.5% Jinggangmycin·3 billion live spores / milliliter bacillus amyloliquefaciens aqueous agent provided by embodiment 1.

[0052] Contrast drugs:

[0053] 5% Jinggangmycin aqueous solution was produced and provided by Tonglu Huifeng Biotechnology Co., Ltd., Zhejiang Province.

[0054] The 6 billion live Bacillus amyloliquefaciens spores / ml water solution is produced and provided by Jiangsu Suke Agrochemical Co., Ltd.

[0055] Indoor synergistic bioassay:

[0056] The rice sheath blight strains to be tested were transplanted on PSA medium (containing 200 grams of potato, 20 grams of sucrose, and 20 grams of ag...

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Abstract

The invention relates to a bacillusamyloliquefaciens and validamycin compound biological bactericide, which comprises an active ingredient and auxiliaries. The bacillusamyloliquefaciens and validamycin compound biological bactericide is characterized in that a bactericide finished product contains 99 weight percent of active ingredient, wherein the active ingredient consists of bacillusamyloliquefaciens Lx-11 and validamycin; the content of a bacillusamyloliquefaciens biocontrol strain Lx-11 in the compound biological bactericide is 3,000,000,000 living bacillusamyloliquefaciens spores per milliliter, and the content of the validamycin in the compound biological bactericide is 2.5 percent. The compound biological bactericide is sprayed onto rice in the growing period of the rice after being diluted, and is used for preventing and controlling rice diseases.

Description

technical field [0001] The invention relates to a compound biological bactericide and application thereof, in particular to a biological bactericide compounded with Bacillus amyloliquefaciens Lx-11 and Jinggangmycin and its application in preventing and treating rice diseases. Background technique [0002] With the rapid development of the national economy and the continuous improvement of people's living standards, higher and higher requirements are put forward for the quantity, quality and safety of agricultural products. Ensuring the supply of pollution-free and high-quality agricultural products and realizing the sustainable use of resources are the biggest challenges facing agricultural production in the 21st century. However, in the past ten years, due to the large-scale planting of late-maturing high-quality and high-yield rice varieties, climate warming and the popularization of light cultivation techniques, the occurrence of rice diseases and insect pests has genera...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P3/00A01N43/16
Inventor 陈志谊张荣胜刘永峰戴秋华
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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