Artificial interspecific hybridization method for ruditapes variegata and ruditapes philippinarum

A technology of Philippine clam and hybridization method, which is applied in fish farming, application, climate change adaptation, etc., can solve the problem of no variegated clam and Philippine clam, and achieve the effect of improving breeding traits

Active Publication Date: 2015-07-01
DALIAN OCEAN UNIV
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AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the artificial interspecific hybridization method between variegated clam and Philippine clam

Method used

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Experimental program
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Effect test

Embodiment

[0015] On July 5, 2013, 3,000 variegated clams were collected in the intertidal zone of Naozhou Island, Zhanjiang. The gonads were relatively full and were about to mature. At the same time, 5,000 Philippine clams were collected by clam harvesting boats in the shallow sea area between the southeast wharf and Naozhou Island. The gonads were well developed and were about to mature.

[0016] The above two clam samples were used as parents, and placed in the cement pond of Zhanjiang Hengxing South Marine Biotechnology Co., Ltd.'s nursery field, and the method of micro-inflation and artificial feeding of Unicellular algae was used to promote ripening. The amount of bait fed is based on the slight greening in the water to ensure that there is always bait in the water. Check whether the parents are dead every day, and check out the dead parents. After 12 days of rearing, the two clam parents matured synchronously.

[0017] When the two are matured synchronously, use a 0.5mm diamete...

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Abstract

The invention discloses a distant hybridization method for ruditapes, in particular to an artificial interspecific hybridization method for ruditapes variegata and ruditapes philippinarum. According to the artificial interspecific hybridization method, a group hybridization mode is adopted, highest-yield artificial interspecific hybridization between the ruditapes variegata and the ruditapes philippinarum is achieved in China, breeding season reproductive isolation obstacles existing between the ruditapes variegata and ruditapes philippinarum are broken through, precious medium materials used for researching little ruditapes genetic evolution and species formation are obtained, a new species with the significant hybrid vigour is obtained at the same time, the foundation is laid for the little ruditapes industry development and genetic improvement, precious materials are provided for little ruditapes genetic evolution and species formation, and the direction is offered to the cultivation of the new species. The artificial interspecific hybridization method for the ruditapes variegata and the ruditapes philippinarum can produce large-scale hybrids, and effectively improve the current cultivations of little ruditapes.

Description

technical field [0001] The invention relates to the technical field of shellfish genetics and breeding in marine agriculture, in particular to an artificial interspecies hybridization method of variegated clams and philippine clams. Background technique [0002] Interspecific hybridization can significantly expand the gene pool of animals and plants, promote interspecific communication, introduce beneficial genes from different species, create unprecedented types of variation, and even synthesize new species. It is one of the basic breeding methods of animals and plants. It is relatively common in the breeding of crops and terrestrial animals, and has achieved relatively good economic benefits and promoted the development of related industries. [0003] For aquatic animals, interspecific hybridization mainly focuses on fish breeding (Luo Yundong, 1999). There are also some related reports on shellfish, mainly oysters (Gaffney & Allen, 1993; Xu et al, 2009; Zhang et al, 2012...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A01K61/54
CPCY02A40/81
Inventor 闫喜武张跃环杨凤张国范
Owner DALIAN OCEAN UNIV
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