Primer, kit and method for conducting genetic typing on hantavirus by means of PCR direct sequencing method
A hantavirus and genotyping technology, applied in the field of genetic diagnosis, to achieve the effect of simple and rapid genotyping
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[0032] 1. Main Reagents
[0033] RNA extraction kit Rneasy Mini Kit was purchased from Qiagen; cloning vector, T4 ligase, M-MLV reverse transcriptase, DNA Taq enzyme, polymerase chain reaction (PCR) related reagents, agarose gel purification kit and plasmid The DNA purification kit was purchased from Japan TaKaRa China Branch.
[0034] 2. Primer design
[0035] The gene sequences of 24 representative strains of Hantavirus were downloaded from the Genbank database. The 24 representative strains of the virus included 7 serotypes. See Table 1 for details.
[0036] Table 124 Information about representative strains of Hantavirus
[0037]
[0038]
[0039] Note: Aa: Apodemus agraius, black-lined rat; Nc: Niviventer confucianus, community mouse; Hu: Human, human; Rr: Rattus rattus, black mouse; Rn: Rattus norvegicus, brown house mouse; Rl: Rattus losea, yellow hair Mouse; Af: Apodemus flavicollis, Apodemus; Cg: Clethrionomys glareolus, European brown-backed rat; Ol: Oligory...
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