New antifungal compositions
一种组合物、抗真菌的技术,应用在杀菌剂、食物科学、杀生物剂等方向,能够解决未观察到抗性真菌物种等问题
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Embodiment 1
[0034] pre-harvest application
[0035] Inoculate the leaves of banana plants with the fungus. Uninoculated leaves were included as controls. Then, use composition 1 (natamycin), composition 2 (azoxystrobin), composition 3 (kresstrobin), composition 4 (phenoxystrobin), composition 5 (trifloxystrobin), Composition 6 (Picoxystrobin), Composition 7 (Pyraclostrobin), Composition 8 (Kysstrobin), Composition 9 (Fluooxastrobin), Composition 10 (Kysstrobin), Composition 11 (natamycin + azoxystrobin), composition 12 (natamycin + azoxystrobin), composition 13 (natamycin + fenoxystrobin), composition 14 (natamycin Composition 15 (natamycin + pyraclostrobin), composition 15 (natamycin + pyraclostrobin), composition 17 (natamycin + pyraclostrobin ), the composition 18 (natamycin + fluoxastrobin) or the composition 19 (natamycin + orysastrobin) to treat a defined part of the leaves. Each composition is applied by spraying. Untreated leaves were also included (untreated control).
[00...
Embodiment 2
[0038] post harvest application
[0039] Bananas were wounded according to the method described by de Lapeyre de Bellaire and Dubois (1987). Bananas were wounded using a cork puncher, after which they were contaminated with fungal spores. After several hours of incubation at room temperature, the bananas were immersed in one of the following compositions: a) no treatment (control 1), b) in water (control 2), c) in natamycin, d) Soaked in Azoxystrobin, e) Soaked in Kresstrobin, f) Soaked in Phenoxystrobin, g) Soaked in Trifloxystrobin, h) Soaked in Picoxystrobin, i) Soaked in Pyrostrobin In pyraclostrobin, j) in kysoxstrobin, k) in fluoxastrobin, l) in oryzastrobin, m) in natamycin + azoxystrobin, n ) soaked in natamycin + kresoxim-methyl, o) soaked in natamycin + fenoxystrobin, p) soaked in natamycin + trifloxystrobin, q) soaked in natamycin + picoxystrobin, r) soaked in natamycin + pyraclostrobin, s) soaked in natamycin + kyrestrobin, t) soaked in natamycin + fluoxastrobin...
Embodiment 3
[0042] handling bananas
[0043] Four organic, unripe (green) bananas were used per treatment group. The skin of each banana was wounded three times using a cork borer according to the method described by de Lapeyre de Bellaire and Dubois (1987). Subsequently, use 15 μl containing 1 x 10 5 Each wound was inoculated with spores / ml of Fusarium proliferatum suspension. After incubation at 20°C for 4 hours, each banana was treated with 100 μl of a freshly prepared aqueous antifungal composition containing 500 ppm natamycin (DSM Food Specialties, Delft, The Netherlands), 1000 ppm fluoxastrobin or both wound. In addition, the antifungal composition comprises 1.00% (w / w) methyl hydroxyethyl cellulose (MHEC), 0.40% (w / w) xanthan gum, 0.20% (w / w) antifoaming agent, 0.30% ( w / w) citric acid, 0.39% (w / w) lactic acid and 0.11% (w / w) potassium sorbate. The pH of the composition is 4. A composition without natamycin and fluoxastrobin was used as a control. The treated, unripe bananas...
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