Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Microrna and its encoding nucleic acid molecule and application related to the regulation of rice leaf width

A technology for encoding nucleic acid and nucleic acid molecules, which is applied in the biological field and can solve problems such as research lag

Inactive Publication Date: 2015-10-14
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although great progress has been made in theoretical research, miRNA also has the potential to provide abundant gene resources for plant genetic improvement, but the current research on its application in production is relatively lagging behind

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microrna and its encoding nucleic acid molecule and application related to the regulation of rice leaf width
  • Microrna and its encoding nucleic acid molecule and application related to the regulation of rice leaf width
  • Microrna and its encoding nucleic acid molecule and application related to the regulation of rice leaf width

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1. Discovery and expression analysis of miRNAs and their coding genes related to regulation of rice leaf width

[0057] 1. Discovery of rice leaf width regulation-related miRNAs and their coding genes

[0058] A reverse genetics study was conducted on multiple rice miRNA genes pre-screened from the japonica rice variety Nipponbare (Oryza sativa L.subsp.japonica cv.Nipponbare). The main research strategy was to analyze the overexpression of the target gene. During phenotypic analysis of transgenic rice populations, a rice leaf width regulation-related miRNA and its coding gene were identified and identified.

[0059] The nucleotide sequence of the miRNA is sequence 1 of the sequence table, and belongs to a miRNA family conserved in plants—miRNA319 family. There are two members of the miR319 family in rice, namely osa-miR319a and osa-miR319b. The sequences of the mature osa-miR319b and osa-miR319a are identical, both consisting of 20 nucleotides (sequence 1 of t...

Embodiment 2

[0076] Example 2, Obtaining and phenotypic analysis of transgenic rice overexpressing Osa-MIR319a and Osa-MIR319b genes and gene fragments

[0077] Theoretically, the biological effects produced by overexpressing miRNA genes and gene fragments with complete stem-loop structure are roughly the same. In order to study the biological function of the rice MIR319 gene, Osa-MIR319aFL (referring to the full-length cDNA of Osa-MIR319a), Osa-MIR319aST (referring to the gene fragment containing the stem-loop structure of Osa-MIR319a), Osa-MIR319bFL (referring to Osa - the full-length cDNA of MIR319b) and Osa-MIR319bST (referring to the gene segment comprising the stem-loop structure of Osa-MIR319b) are respectively placed under the control of the Actin of rice or the Ubi promoter of corn to construct a binary vector ( Figure 5 ), and transformed into rice indica variety Kasalath (Oryza sativa L. subsp. indica cv. Kasalath).

[0078] 1. Preparation of recombinant plasmids

[0079] 1. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a rice leaf width adjustment and control related microRNA (ribonucleic acid) as well as an encoding gene and application thereof. The RNA provided by the invention is one of 1)-4), namely 1) RNA consisting of a nucleotide sequence shown in the sequence 1 of a sequence table, 2) RNA consisting of a nucleotide sequence shown in the sequence 4 of the sequence table, 3) RNA consisting of a nucleotide sequence shown in the sequence 5 of the sequence table and 4) RNA which is substituted and / or lost and / or added from the RNA sequence shown in 1) or 2) or 3) through one or more basic groups, is related to plant leaf width, and is derived by 1) or 2) or 3). Experiments prove that Osa-miR319 and encoding gene thereof are discovered, the width of rice leaves can be effectively adjusted and controlled by utilizing the gene through a genetic engineering method, and the leaf shapes of rice can be changed.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a microRNA related to the regulation of rice leaf width and its encoding nucleic acid molecule and application. Background technique [0002] MicroRNA (miRNA) is a class of endogenous non-coding RNA with regulatory functions found in eukaryotes and viruses, and its size is 19-24 nucleotides (nucleotides, nt). In plants, miRNAs are mostly encoded by independent genes. In the nucleus, miRNA genes are first transcribed into longer transcripts by RNA polymerase II, which are called primary miRNA transcripts (primary miRNA, pri-miRNAs). The structure of primary transcripts is similar to general mRNA, and most of them also have 5'-cap and 3'-poly(A) tail structures. Primary transcripts contain imperfectly matching stem-loop structures. Primary transcripts can be processed by the RNaseIII family enzyme Dicer like1 (DCL1) and some other related double-stranded RNA-binding proteins into mi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/11C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21A01H5/00
Inventor 朱立煌李大勇杨春花刘雪
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com