Culture medium for producing oxytetracycline through streptomyces rimosus fermentation and fermentation method

Abstract

The invention relates to a culture medium for producing oxytetracycline through streptomyces rimosus fermentation and a fermentation method. According to the invention, by replacing conventional soybean cake powder with low-temperature soybean cake powder, stopping animal protein substances and optimizing the formula of the culture medium, the problem in the cost of the raw and accessory materials is solved, the environmental influence on the source of the raw and accessory materials is minimized, sufficient supply is guaranteed, and stable and efficient production of oxytetracycline is realized. Meanwhile, by adopting the culture medium and method provided by the invention to produce oxytetracycline, the fermentation unit of oxytetracycline can be increased to over 34000u / ml, which is increased by over 6% in comparison with the domestic conventional technologies; and compared with the prior art, the fermentation period is shortened by over 5%, the oxytetracycline output is increased, the production efficiency is improved, and the aim of saving energy and reducing consumption is achieved.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium and a fermentation method for producing oxytetracycline by fermentation of Streptomyces crackis. Background technique [0002] Oxytetracycline is a broad-spectrum antibiotic, which has inhibitory effects on Gram-positive bacteria and Gram-negative bacteria, and also has certain inhibitory effects on Chlamydia, Rickettsia, Mycoplasma, Spirochetes, etc. Oxytetracycline is mainly used to prevent and treat Escherichia coli, Salmonella infection, Pasteurella, Brucella infection, swine panting disease, and chicken chronic respiratory disease; it is also commonly used to treat dog rickettsiasis and cat infectious Anemia, prevention of chlamydial disease and leptospirosis in dogs; locally used for endometritis and tissue necrosis caused by Necroptosis bacillus; used as a feed lick additive to promote growth and improve feed conversion rate. [0003] Acc...

AI Technical Summary

Problems solved by technology

Peanut cake powder and cottonseed cake powder are used as the nitrogen source of the fermentation medium. Due to their poor quality and high content of impurities, which affect the metabolites of the strains, the color of the fermentation broth is darkened, and the fermentation unit is low. The fermentation unit is generally 20,000 to 23,000. Ultimately affect product quality; if the proportion of animal protein compatibility is high, a large amount of foam will be generated during the fermentation process. If it is not strictly controlled, the fermentation liquid will escape and cause bacterial contamination

At the same time, due to the high viscosity of the fermentation broth, it has a great influence on the yield of post-refining

[0005]2 The organic nitrogen source animal protein added by oxytetracycline fermentation culture, such as fish meal and fat extract, due to the influence of market conditions and seasons, the purchase cost and stressful

The fermentation cycle is long and the energy consumption is high; the process control is more complicated, especially the temperature, stirring speed, feeding and pH control parameters have a great influence on the fermentation unit and the volume of the fermentation broth