Rooting method of subprostrate sophora root tissue culture seedling leaf stalks
A petiole and tissue culture technology is applied to the rooting field of the petioles of the tissue culture seedlings of broad bean root, and can solve the problems of serious pests and diseases, easy decline of vigor, low seed yield and the like.
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[0012] The technical solutions of the present invention will be further described below in conjunction with the examples.
[0013] A method for rooting the petiole of broad bean root tissue culture seedling, comprising the following steps:
[0014] (1) Cultivation of strong seedlings of group-cultured clustered shoots: Take broad bean root tissue-cultured clustered shoots and inoculate them into MS strong seedling medium, at a culture temperature of 23-27°C, with a light intensity of 1500 lux and a light time of 12-14 hours / Cultivate for 30 days under the condition of 1 day, and the leaves will expand after the lignification of the clustered buds, and the compound leaves with stalks with three leaves will be obtained, wherein the MS strong seedling medium contains 1.0mg / L of 6-BA and 0.1mg / L of NAA , the IBA of 0.5mg / L, the agar of 30g / L sucrose and 3.4g / L, the pH value of culture medium is 5.8;
[0015] (2) rooting culture of tissue culture seedling petiole: the compound le...
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