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Talaromyces flavus and application of same in prevention of plant pathogens

A yellow taromycetes, plant pathogenic bacteria technology, applied in the application, plant growth regulators, botanical equipment and methods, etc., can solve problems such as serious disease occurrence, environmental pollution, etc., to improve quality and yield, prevent environmental pollution, Significant economic and social benefits

Active Publication Date: 2013-05-29
QINGDAO AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problems of environmental pollution and serious disease occurrence in chemical pesticide prevention and control in the prior art, the purpose of the present invention is to provide a yellow basket-shaped fungus that can effectively prevent and control a variety of crop pathogens, and also relates to its ability to inhibit a variety of pathogens. Applications

Method used

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  • Talaromyces flavus and application of same in prevention of plant pathogens
  • Talaromyces flavus and application of same in prevention of plant pathogens
  • Talaromyces flavus and application of same in prevention of plant pathogens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Isolation of antagonistic bacteria

[0029] The study found that the peanut rhizosphere soil after 4 years of continuous cropping in Laixi City, Shandong Province, compared with the peanut rhizosphere soil of 2 and 7 years of continuous cropping, the species and number of plant pathogens were significantly reduced, the obstacles to continuous cropping of peanuts were significantly reduced, and the yield and quality were improved. The rhizosphere soil of peanuts in continuous cropping for 4 years in Laixi City, Shandong Province was separated by dilution separation method. The yellow-blue bacteria described in the present invention are isolated from the rhizosphere soil of peanuts in continuous cropping for 4 years. By purifying the single spores of the isolates, they are inoculated with common plant pathogenic bacteria at two points on the PDA plate respectively, and the plate antagonism test is carried out. Filtered to get.

Embodiment 2

[0030] Embodiment 2, identification of antagonistic bacteria

[0031] 1. Morphological identification of antagonistic bacteria

[0032] The medium used in the experiment is as follows:

[0033] (1) Chase agar (CA): 3.0g sodium nitrate, 1.0g dipotassium hydrogen phosphate, 0.5g potassium chloride, 0.5g magnesium sulfate heptahydrate, 0.01g ferrous sulfate heptahydrate, 30g sucrose, 15g agar, distilled water 1000mL, heated to dissolve, and finally adjusted to 1000mL, sterilized at 121°C for 15min.

[0034] (2) Chabai Yeast Extract Agar (CYA): 10mL of Chabai concentrated stock solution, 1.0g of dipotassium hydrogen phosphate, 5g of yeast extract, 30g of sucrose, 15g of agar, 1000mL of distilled water, heat to dissolve, and finally dilute to 1000mL. Sterilize at 121°C for 15 minutes.

[0035] Cha's concentrated stock solution: 30g of sodium nitrate, 5g of potassium chloride, 5g of magnesium sulfate heptahydrate, 0.1g of ferrous sulfate heptahydrate, 100mL of water, heated to di...

Embodiment 3

[0055] Embodiment 3, the bacteriostasis of yellow basket bacterium.

[0056] The yellow basket fungus was inoculated with plant pathogenic fungi such as Chestnut Phytophthora, Curvularia maize leaf spot, Wheat Scab, Tobacco Alternaria Alternaria, Poplar Rot and Rhizoctonia solani and other plant pathogenic fungi using a two-point confrontation method. At the same time, the PDA plate inoculated with each pathogenic bacteria was used as a control. Place in a constant temperature and humidity incubator at 28°C for culture, and measure the colony diameter 3 days after inoculation until the control plate is covered with the plate. The antibacterial rate was calculated, and the results are shown in Table 1.

[0057]

[0058]

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Abstract

The invention provides a talaromyces flavus, and also relates to the application of the talaromyces flavus in the inhibition of a variety of plant pathogens. The preservation number of the talaromyces flavus qw12 is CGMCC5067, the talaromyces flavus colony is round, the texture is flocculent and fluffy or flocculent, and the front of the colony is yellow; the conidiophores have penicilli, and the spore is spherical; the primordium is nearly cylindrical, big and curved; the gymnothecium is spherical or nearly spherical, the ascus is spherical, and the ascospore is spherical or nearly sphericaland unicellular. The antagonistic bacterium provided by the invention can effectively inhibit the growth of valsa sordida, rhizoctonia solani, corn sheath blight fungi, curvularia lunata, colletotrichum gloeosporioides, fusarium graminearum, alternaria alternate, cryphonetria parasitica, colletotrichum nicotianae and other plant pathogens, effectively prevent plant diseases, reduce the dosage of chemical pesticide and the accumulation of the chemical pesticide in soil and help to increase the quality and yield of economic crops in China, and therefore economic and social benefits are remarkable.

Description

technical field [0001] The invention relates to the field of microorganisms and biological control, in particular to a yellow basket fungus and its application in preventing and treating plant pathogenic bacteria. Background technique [0002] At present, the general method of controlling plant diseases is to use chemical pesticides. However, long-term use of chemical pesticides will lead to resistance of pathogenic bacteria and reduce the disease prevention effect of chemical agents. At the same time, long-term use of chemical agents will cause excessive pesticide residues and environmental pollution. Biological control is a new way to control plant diseases. It mainly uses the antagonism between biocontrol bacteria and plant pathogenic microorganisms to inhibit the growth of plant pathogenic bacteria. Peanut is the main economic crop in my country, and its output and quality are related to the national economy and people's livelihood. In recent years, due to the increasin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05F11/08A01N63/04A01P3/00C12R1/645
Inventor 咸洪泉颜艳伟崔德杰王大伟柳新伟
Owner QINGDAO AGRI UNIV
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