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Application of bdellovibrio sp. leech plasmid bacterium solution in cultivating penaeus monodon

A technology of Penaeus monodon and Bdellovibrio, which is applied in the fields of application, bacteria, and fish farming, can solve the problems of adverse effects on the immunity of shrimp organisms, drug resistance, drug residues, and slow effect, so as to improve the disease resistance of shrimp ability, prevention of vibriosis in prawns, and the effect of improving the survival rate

Inactive Publication Date: 2013-04-17
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese herbal medicine has the advantages of no drug residue and high economic benefit on the growth performance of Penaeus monodon, but has the disadvantage of slow effect
Antibiotics have played a positive role in inhibiting the reproduction of pathogenic microorganisms and reducing the toxicity of their metabolic toxins to shrimp. However, the long-term use of antibiotics, especially the abuse of antibiotics, has gradually revealed its various disadvantages
Such as destroying the natural ecological balance in shrimp ponds, adversely affecting the immunity of the shrimp body, producing side effects such as drug resistance and drug residues, and bringing potential harm to the health of the aquaculture industry and human beings as consumers.

Method used

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  • Application of bdellovibrio sp. leech plasmid bacterium solution in cultivating penaeus monodon
  • Application of bdellovibrio sp. leech plasmid bacterium solution in cultivating penaeus monodon
  • Application of bdellovibrio sp. leech plasmid bacterium solution in cultivating penaeus monodon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation of Bdellovibrio Budoplasma Bacteria Liquid

[0024] The said hirudin is obtained by the following method: in the presence of Vibrio parahaemolyticus (inoculation concentration of 10 7 cfu / mL bacteria 1mL) (Vibrio parahaemolyticus, purchased from American Type Culture Collection, ATCC 17802) 1L DNB (dilute nutrient broth) liquid medium (nutrient broth 0.8g, casein acid hydrolysate 0.5 g, 0.1g yeast extract, dissolved in 1000mL distilled water, pH value is 7.6) connected to 1mL concentration of 10 3 pfu / mL Bdellovibrio, cultured at 28℃, 250rpm for 48h, the culture solution was centrifuged at 4℃ 6000rpm for 15min, then the supernatant was removed, and the precipitate was suspended in 100mL DNB to obtain the budoplasm; Bdellovibrio BDFM05 The concentration reaches 10 9 pfu / mL.

Embodiment 2

[0025] Example 2 Application experiment of Bdellovibrio Budoplasma bacteria liquid in common culture of Penaeus monodon

[0026] The specific implementation site of the present invention is a breeding farm in South China, and the implementation time is 3 months.

[0027] (1) Conditions of the breeding pond, the bottom area of ​​the breeding pond is 10 mu, the depth of the pond is 2m, the bottom of the pond is sandy, the bottom of the pond is flat, the breeding pond is equipped with pumps, aerators, and equipped with pumping station gates and relatively complete drainage system;

[0028] (2) Preparations before stocking. The water quality of the water source in the sea area of ​​the test area meets the requirements of the National Standard Fishery Water Quality Standard of the People’s Republic of China (GB11607-89). The sea water enters the aquaculture pond after sand filtration and sedimentation treatment, which meets the requirements of the Ministry of Agriculture. Water quality f...

Embodiment 3

[0111] Example 3 Application experiment of Bdellovibrio budoplasma in high pond culture of Penaeus monodon

[0112] (1) The structure of the shrimp pond is 88m×83m×2.2m, the four corners are rounded, and the area of ​​each shrimp pond is 10 mu, arranged horizontally in the east-west direction. The distance between the shrimp ponds is 5m, and the altitude is 10m. Cement concrete slopes are used around the shrimp ponds, plastic film is laid on the bottom of the pond, and then 35cm of sand is covered on the film.

[0113] Dig a well at the lowest low tide line in the sea area, lay the pipeline to the pump, the room distance is 72m, using 100cm concrete pipe; the pump room uses 3 pumps of model 8BA18, power 22kW / set, pumping water to the shrimp pond, the distance is 110m, using 60cm×70cm open channel, and then through 40cm×60cm open channel to divert water into each shrimp pond. It adopts waterwheel type aerator with a power of 1.1kW / unit, and one unit is placed per mu of water surfac...

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Abstract

The invention in particular relates to an application of a bdellovibrio sp. leech plasmid in cultivating marine products such as penaeus monodon, and the like. The invention is realized by adding a bdellovibrio sp. leech plasmid to the water body and the feeds. The invention is realized specifically based on cultivation pond conditions, preparation before stocking, shrimp postlarvae selecting andstocking, feeding management and water environment management in the cultivation period. The invention is characterized by adding a bdellovibrio sp. leech plasmid bacterium solution to the water bodyand ensuring the concentration of the bdellovibrio sp. leech plasmid in the water body to be 101-107pfu / mL, simultaneously soaking the feeds in the bdellovibrio sp. leech plasmid bacterium solution with the concentration of 101-107pfu / mL for 30 minutes and changing water once every 30 days in the cultivation period. The invention provides a method for cultivating organisms. The method has the advantages of environment friendliness, safety, no pollution, obvious effect and capabilities of improving the water quality, promoting growth of penaeus monodon, improving the immunity and improving thesurvival rate, thus having wonderful development prospect. The invention has very important significance in improving the economic benefits and eating safety and guaranteeing human health.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of bacteria, and particularly relates to the application of the Budovibrium bdeltoid bacterium liquid in the breeding of marine products such as Penaeus monodon. Background technique [0002] Penaeus monodon (Penaeus monodon) is commonly known as ghost shrimp, grass shrimp, flower shrimp, bamboo prawn, tiger prawn, and ox-shaped prawn. It is the largest individual of the shrimp. Mature individuals generally have a body length of 22.5cm~32cm and a weight of 137g~211g. It is one of the common species of shrimp farming in the world today. [0003] The current non-sewage farming method causes prawn excrement, residual feed, plankton residues and other organic wastes to accumulate in the pond. In the middle and late stages of aquaculture, sediment pollution will inevitably occur, and the quality of the water body will decline. The increase of germs and pathogenic bacteria, the det...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A23K1/18A23K1/16C12N1/20C12R1/01A23K10/18A23K50/80
CPCY02A40/81
Inventor 蔡俊鹏王沛贤
Owner SOUTH CHINA UNIV OF TECH
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