Freezed shrimp meat quality grading method
A technology of quality grading and quality grading, applied in biochemical equipment and methods, microbiological measurement/inspection, food testing, etc., can solve problems such as no series of indicators proposed by research, no national standards for quality evaluation of frozen shrimp, etc.
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[0006] Assay steps of the present invention can be carried out with reference to the following process:
[0007] 1) Take a sample (thawing is not required).
[0008] 2) Extraction and determination of sarcoplasmic protein:
[0009] Extraction: 5g frozen shrimp (do not need to deglaze), add 30ml pre-cooled 50mM Tris-HCl (pH=7.5) buffer solution, homogenize 3 times with a homogenizer at 15000rpm, 1min each time, homogenize each time After that, wait for 30 seconds (add ice to the periphery from time to time to keep the homogenate at a low temperature). The obtained homogenate was centrifuged with a refrigerated centrifuge (4°C, 10800 rpm, 10 min), and the obtained supernatant was the sarcoplasmic protein solution, also used as the LDH crude enzyme solution. Put it in a brown bottle and store it in a 4°C refrigerator for later use. The determination should be carried out in time, no more than two days.
[0010] Determination: Select the LDH crude enzyme solution stock solutio...
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