Platelet derived growth factor (PDGF)-derived neurospheres define a novel class of progenitor cells
a neural progenitor cell and platelet-derived growth factor technology, applied in the direction of growth factor/regulator, nervous system cells, animal/human proteins, etc., can solve the problem of inability of these cells or the brain region to carry out the intended function
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
example 1
PDGF Induces Production of Primary Neurospheres
[0085]E14 striatum was mechanically dissociated as previously described (for example see U.S. Pat. No. 5,750,376; 5,980,885; or 5,851,832) and plated at 10,000 cells / ml in 6 well plates in defined culture media plus 100 ng / ml of PDGF-AA (Peprotech). The composition of defined culture media is as follows:[0086]DMEM / F12 (1:1)[0087]glucose (0.6%)[0088]glutamine (2 mM)[0089]sodium bicarbonate (3 mM)[0090]HEPES (5 mM)[0091]insulin (25 μg / ml)[0092]transferrin (100 μg / ml)[0093]progesterone (20 nM)[0094]putrescine (60 μM)[0095]selenium chloride (30 nM)
[0096]Neurospheres, which are clusters of neural cells derived from single cells, formed after 7 days of culture in vitro (DIV). Six wells per experiment were counted for neurosphere production and the results are shown below:
Average number of Experiment#neurospheres produced / well114.529.2334.3*48.657.7631.5*average = 17.7 ± 5.0*PDGF appears to be approximately 3 fold more potent when used immedia...
example 2
Tyrphostin AG 1296 Inhibits the Production of Primary Neurospheres by PDGF but not EGF
[0098]In order to determine if the PDGF-induced primary neurosphere formation is mediated by the PDGF receptor kinase, a selective PDGF receptor kinase inhibitor, Tyrphostin AG 1296, was added to neurosphere culture. Primary cells were cultured at 10,000 cells / ml in the presence of either EGF or PDGF, plus 5 μM of Tyrphostin AG 1296 (Sigma) or DMSO in the same volume as Tyrphostin (DMSO being the solvent for Tyrphostin). Results are expressed below as the percentage of neurospheres formed, with the number of PDGF- or EGF-derived neurospheres arbitrarily set at 100%, respectively'.
TABLE 1The Effect of Tyrphostin on neurosphere formation in response to EGF or PDGFPDGF or EGF alonewith DMSOwith TyrphostinPDGF100% 97%10%EGF100%101%55%n = 3 to 9
[0099]Therefore, Tyrphostin AG 1296 almost abolished primary neurosphere formation induced by PDGF, indicating that formation of the PDGF-derived neurospheres is...
example 3
PDGF-Derived Primary Neurospheres can be Subcultured into EGF but not PDGF Containing Media
[0100]Single PDGF primary neurospheres prepared as described in Example 1 were transferred into 96-well plates and mechanically dissociated in either the presence of 20 ng / ml EGF (Peprotech) or 100 ng / ml PDGF-AA. The formation of secondary neurospheres was assayed after 7 or more days in culture in vitro. The results are shown below.
Average # of secondary neurospheres formed / wellPDGF0EGF3.12 ± 1.64 (n = 3)
[0101]Therefore, the cells in PDGF-derived primary neurospheres cannot proliferate in response to PDGF. In contrast, these cells can proliferate and form secondary neurospheres in response to EGF.
PUM
Property | Measurement | Unit |
---|---|---|
cell density | aaaaa | aaaaa |
pharmaceutical composition | aaaaa | aaaaa |
size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com