Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method and application of identification of pharmacodynamic components of traditional Chinese medicine based on target component knockout

A technology with target components and efficacy, applied in the field of pharmaceutical technology and drug screening, it can solve the problems of high throughput and high precision, which are difficult to take into account the overall and systematic views of traditional Chinese medicine.

Active Publication Date: 2022-08-02
FUJIAN HEALTH COLLEGE
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the existing separation and analysis technology is difficult to take into account the holistic view and the systematic view of traditional Chinese medicine in terms of high throughput and high precision, the inventors provide a method for the identification of pharmacodynamic groups of traditional Chinese medicine based on the knockout of target components. The method, technical scheme is as follows:

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and application of identification of pharmacodynamic components of traditional Chinese medicine based on target component knockout
  • Method and application of identification of pharmacodynamic components of traditional Chinese medicine based on target component knockout
  • Method and application of identification of pharmacodynamic components of traditional Chinese medicine based on target component knockout

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Preparation of Indigo Naturalis Extract Sample Powder

[0041] Accurately weigh six parts of Indigo Naturalis powder, 25 g each, and place them in a beaker. Measure 300 ml of N,N-dimethylformamide (DMF) and soak them for 30 minutes. The ultrasonic power is 700 W, the ultrasonic temperature is 50 °C, and the ultrasonic time is 60 minutes. , the ultrasonic extraction times were three times, collect the indigo naturalis extract, filter it, transfer it to a rotary evaporation bottle, and press the rotation speed of 140 r min. -1 , the heating temperature of the water bath is 65 ℃, and the cooling temperature is -4 ℃ to concentrate, and the indigo naturalis extract concentrate is placed in a vacuum oven to prepare powder.

[0042] The sample powder obtained from the experiment is about 2.2 g, and the calculated yield is about 1.47%.

[0043] 2. Preparation of knockout components of Qingdai extract

[0044] 1. Qualitative analysis by semi-preparative liquid chromatograph...

Embodiment 2

[0081] Preparation of Qingdai extract powder: ultrasonic extraction of chemical components in Qingdai, ultrasonic conditions: power 700 W, temperature 50 °C, time 60 min, times 3 times; the extract was concentrated, and the conditions were 140 r min. -1 , the heating temperature of the water bath is 65 °C, and the cooling temperature is -4 °C;

[0082] Preparation of knockout components of Qingdai extract: The target components indirubin, tryptamine and isorhamnetin were separated by semi-preparative liquid chromatography. Chromatographic conditions: Shim-pack GISS C18 column was used with a flow rate of 3 mL min -1 , the detection wavelength was 290 nm, and the sample volume was 4 mL; elution conditions: gradient elution was performed with 0.1% glacial acetic acid-methanol as the mobile phase. The elution procedure is shown in Table 1. like image 3 Indirubin, tryptamine, and isorhamnetin-free fractions (ABC-) were collected from 0.33 min to 57.5 min, 64.01 min to 75.0 min...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method and application for identifying medicinal effect components of traditional Chinese medicines based on knockout of target components, and belongs to the fields of pharmaceutical technology and drug screening. The method includes extracting the chemical components of traditional Chinese medicine, knocking out target components to prepare different component groups, identifying the knock-out components to evaluate the knock-out effect, quantifying the ratios of different component groups in the traditional Chinese medicine that are actually restored by the knock-out components, and determining the main pharmacodynamic component groups. The invention prepares and screens out the knockout component of the Qingdai extract with anti-leukemia effect. It provides basic support for the follow-up quality control of Indigo Naturalis, anti-leukemia efficacy research and pharmaceutical preparations, and also has reference and promotion value for other drugs.

Description

technical field [0001] The invention belongs to the field of pharmaceutical technology and drug screening, in particular to a method for identifying or screening medicinal effect components of traditional Chinese medicines based on the combination of target knock-out components and quantitative methods, and its application in anti-leukemia of Indigo Naturalis. Background technique [0002] Leukemia is a malignant disease of the hematopoietic system, and is one of the top ten malignant tumors that cause the death of Chinese residents. Under the guidance of traditional Chinese medicine theory, the traditional Chinese medicine compound "Danggui Longhui Pill" and "Huangdai Tablet" can effectively treat leukemia. Indigo Naturalis in the above-mentioned compound is the most important anti-leukemia Chinese herbal medicine. It is the leaves or stems of Aphicacanthuscusia (Nees) Bremek, Polygonum tinctonum Ait., or Isatisindigotica Fort. The processed dry powder or agglomerate has t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/146
Inventor 黄慧李泳宁戴雅彬张月芬
Owner FUJIAN HEALTH COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com