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Fluorescence-encoded microspheres and arrays and preparation methods

A technology for encoding microspheres and microspheres, which is applied in the preparation of microspheres, chemical instruments and methods, and microcapsule preparations. It can solve the problems of encoding capacity limitations, achieve fast reaction kinetics, not easily settle, and improve detection sensitivity.

Active Publication Date: 2021-11-23
SHANGHAI MAG GENE NANOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has the advantages of simple operation and stable fluorescence, it also limits the functional groups on the surface of the microspheres, and only uses the outer surface of the microspheres to label fluorescent molecules, which greatly limits the coding capacity.

Method used

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  • Fluorescence-encoded microspheres and arrays and preparation methods
  • Fluorescence-encoded microspheres and arrays and preparation methods
  • Fluorescence-encoded microspheres and arrays and preparation methods

Examples

Experimental program
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Embodiment 1

[0164] In the encoded microspheres of this embodiment, a group of microsphere carriers used have similar diameters and different internal structures, and the internal structure changes of the microsphere carriers are used as the first dimension of encoding information; wherein the microsphere carriers are mesoporous microspheres , which has a porous structure, and different internal structures may be due to different matrix components, different pore sizes, or different matrix components and pore sizes.

[0165] The selection range of the diameter of the microsphere carrier is 0.2-20 μm, and the preferred range is 3-6 μm. The pore diameter of the microsphere carrier can be selected within the range of 2-100 nm, preferably within the range of 10-60 nm. The matrix components of microsphere carriers include inorganic substances and polymers. Inorganic substances include silica and / or titania. Polymers include polystyrene, polyacrylic acid, polymethyl acrylate, polymethacrylic a...

Embodiment 2

[0255] As mentioned above, according to the preparation method of Example 1, step 5 can be repeated to assemble multiple layers of quantum dots with different central emission wavelengths on the outer surface of the microsphere carrier, thereby further increasing the dimension of the encoding and amplifying the encoding. quantity.

[0256] Similarly, on the basis of the preparation method in Example 1, two or more fluorescent dyes with different central emission wavelengths can also be loaded inside the microsphere carrier. The focus of this embodiment is to further describe in detail the preparation method of encoded microspheres loaded with one or more fluorescent dyes inside the microsphere carrier. The encoded microspheres in this embodiment can be used alone, or this The method of the embodiment is applied in step 2 of the first embodiment, thereby enlarging the encoding dimension of the encoded microspheres in the first embodiment.

[0257] The design of the preparation...

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Abstract

The present invention provides a fluorescent coded microsphere, an array and a preparation method, wherein the coded microsphere includes a microsphere carrier, the microsphere carrier is a mesoporous microsphere, and at least one kind of fluorescent light is arranged inside the microsphere carrier. Markers, the fluorescent markers are formed by connecting fluorescent dyes with intermediary substances, and the central emission wavelengths of the fluorescent dyes corresponding to each of the fluorescent markers are different, so that each fluorescent dye defines one of the encoded microspheres Encoding information for the dimension.

Description

technical field [0001] The invention relates to the field of micro-nano biological materials and multi-index detection, in particular to a fluorescent coded microsphere, an array and a preparation method. Background technique [0002] The suspension array technology based on coded microspheres is one of the multi-index detection technologies that have received wide attention in recent years. Identification and quantitative analysis have important application value in the fields of disease diagnosis, drug development, and environmental pollution monitoring. Compared with the traditional solid-phase chip, the suspension array technology replaces the flat plate carrier with coded microspheres with micro-nano scale, and each type of microsphere carries a coded information that can be recognized by the instrument to identify the probes immobilized on its surface Species, after specifically reacting with the corresponding target molecule, the resulting complex has a detection sig...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C09K11/02C09K11/88B01J13/02
CPCC09K11/06C09K11/883C09K11/025B01J13/02C09K2211/1088
Inventor 王耀徐宏古宏晨
Owner SHANGHAI MAG GENE NANOTECH CO LTD
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