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A kind of anti-drug-resistant fungi pharmaceutical composition and preparation thereof

A technology of antifungal drugs and compositions, applied in the field of medicine, can solve the problems of aggravating the drug resistance of Candida albicans to fluconazole, and achieve the effect of anti-drug-resistant fungi

Active Publication Date: 2022-05-27
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During long-term treatment, repeated administration aggravates the occurrence of fluconazole resistance in Candida albicans, and most fungi that are resistant to fluconazole also have cross-resistance to other antifungal drugs. A large number of drug-resistant strains have emerged, and the current antifungal drugs have fallen into a bottleneck in the treatment of fungal infectious diseases

Method used

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  • A kind of anti-drug-resistant fungi pharmaceutical composition and preparation thereof
  • A kind of anti-drug-resistant fungi pharmaceutical composition and preparation thereof
  • A kind of anti-drug-resistant fungi pharmaceutical composition and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Wash the screen edge Panax notoginseng, dry it, and grind it into coarse powder. Take 1100g of coarse powder, add 95% ethanol to reflux for 2 times, the first time is 2h, the second time is 1.5h, and the dosage of 95% ethanol is 22L each time. After refluxing, filter, combine the filtrates, and obtain 342.8 g of extract after vacuum distillation. Dissolve the extract in 1L of water, extract 4 times with petroleum ether and n-butanol respectively, and the amount of solvent for each time is 0.3L; combine the n-butanol extracts and distill under reduced pressure to obtain the n-butanol part extract: 151.90g, That is, Pingbian Panax notoginseng n-butanol extract.

Embodiment 2

[0059] (1) Wash the screen edge Panax notoginseng, dry it, and grind it into coarse powder.

[0060] (2) Coarse powder is refluxed with ethanol for 2 times, the dosage of each time is 5-15 times the mass volume (kg / L) of Pingbian Panax notoginseng coarse powder, 1~3 hours each time, filtered after reflux, combined filtrate, reduced pressure Concentrating the extract until there is no alcohol smell to obtain Pingbian Panax notoginseng ethanol extract;

[0061] (3) Suspend the Pingbian Panax notoginseng ethanol extract with water, extract with petroleum ether and n-butanol in turn, and extract 3-6 times with each solvent; combine the n-butanol extracts, and concentrate the extracts under reduced pressure until there is no alcohol smell. Pingbian Panax notoginseng n-butanol extract was obtained.

[0062] The mass concentration of ethanol described in step (2) is 80-90%; the amount of water used for suspension described in step (3) is 1-3 times that of Pingbian Panax notoginseng ...

Embodiment 3

[0073] Detection of anti-drug-resistant Candida albicans in vitro

[0074] 1-fold ratio microdilution method

[0075] Dissolve 0.1 g of Pingbian Panax notoginseng n-butanol extract in 1 mL of dimethyl sulfoxide (DMSO) to form a storage solution with a concentration of 100 mg / mL, and store at 4°C; take a fluconazole dispersible tablet and grind it into powder. Add 1 mL of DMSO to prepare a stock solution with a concentration of 50 mg / mL, and store at 4°C.

[0076] Take the prepared Pingbian Panax notoginseng n-butanol extract stock solution (100mg / mL) and fluconazole stock solution (50mg / mL) and dilute to 1mg / mL and 0.5mg / mL respectively, as the initial concentration, use Micro-dilution method was used for 5-fold dilution, and 6 concentration gradients were set for each experiment [19] , set up Pingbian Panax notoginseng n-butanol extract single-use group, fluconazole single-use group and Pingbian Panax notoginseng n-butanol extract combined with fluconazole group, respective...

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PUM

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Abstract

The invention belongs to the technical field of biomedicine and discloses a pharmaceutical composition capable of resisting drug-resistant fungi, which is composed of panax notoginseng n-butanol extract and antifungal drugs. Panax notoginseng is washed, dried and pulverized, extracted with n-butanol, and obtained after vacuum distillation to obtain the n-butanol extract of Panax notoginseng. Pingbian notoginseng n-butanol extract can significantly reduce the efflux function of fungal cell efflux pump, significantly down-regulate the expression level of drug resistance-related genes and reduce the mitochondrial membrane potential level in fungal cells; Pingbian notoginseng n-butanol extract The pharmaceutical composition composed of antifungal drugs has good inhibitory effect on drug-resistant fungi.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to an anti-drug-resistant fungal medicinal composition and its preparation, which are composed of Pingbian Panax notoginseng n-butanol extract and antifungal medicine. Background technique [0002] Candida albicans ( Candida albicans ) is an opportunistic pathogenic fungus that exists in human skin, mucous membranes, etc., and generally does not cause disease in normal organisms. In recent years, due to the large-scale application of radiotherapy and chemotherapy in cancer patients, the large-scale application of immunosuppressive agents in organ transplant patients, and the large-scale use of broad-spectrum antibiotics, the incidence of systemic Candida albicans infection has increased rapidly, with an infection rate as high as 46.36%. [0003] At present, there are few drugs that can be used for clinical anti-deep infection of Candida albicans, mainly including ampho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/258A61P31/10A61K125/00
CPCA61K36/258A61P31/10A61K2236/333A61K2236/39A61K2236/53A61K2236/51
Inventor 王睿睿其他发明人请求不公开姓名
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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