Device and method for detecting sweetness solution
A detection method and sweetness technology, applied in the field of solution detection, can solve the problems of repeatability limitations, repeatability and accuracy limitations, and inconvenient operation of the instrument, so as to eliminate interference factors, reduce interference, and improve detection timeliness Effect
Active Publication Date: 2022-05-31
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology
Although the patch clamp method can detect the ion channel response signals of single cells, the instrument is inconvenient to use and can only be used in the laboratory
Although the bionic taste detection technology can realize the classification of taste substances to a certain extent, its repeatability and accuracy are limited.
Although the electrochemical cell sensor method can achieve the detection target of taste substances to a certain extent, its repeatability is also limited.
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[0066] The infusion device includes a microperistaltic pump. The axis of the first guide groove and the axis of the second guide groove are at the same line
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Abstract
The invention discloses a sweet solution detection device and method. The device includes a computer, a phase contrast microscope, infusion equipment, and a placement module for placing taste receptor cells. The placement module includes a slide, and the upper surface of the slide is provided with a placement groove for placing taste receptor cells. The two sides of the upper surface of the slide are respectively provided with a liquid inlet and a liquid outlet, and the upper surface of the glass slide is also provided with a first diversion groove connecting the liquid inlet and the placement groove, and a second diversion groove connecting the liquid outlet and the placement groove , the liquid inlet is connected with the infusion equipment through the pipeline, the liquid outlet is connected with the waste liquid collector through the pipeline, and the phase contrast microscope is electrically connected with the computer. The invention uses the method of cell imaging without contact with the cells to be tested, greatly reduces the interference brought by the outside world, eliminates the interference factors affecting the instantaneous physiological state of the cells, and has fast detection timeliness and high detection accuracy.
Description
A kind of sweet solution detection device and method technical field The present invention relates to the technical field of solution detection, in particular to a kind of sweet solution detection device and method. Background technique Taste cell sensor is a kind of cell sensor, which uses taste receptor cells as molecular recognition elements, which can To determine the existence and concentration of a certain type of taste (such as sweet, bitter, salty, sour, etc.), so as to achieve qualitative and quantitative Test analysis, and the extent of real-time, fast and non-destructive intelligent inspection. [0003] The current detection methods include patch clamp method, bionic taste detection method, electrochemical cell sensor method. patch clamp Although the method can detect the ion channel response signal of single cell, the instrument is inconvenient to use and can only be used in Laboratory use. Although bionic taste detection technology can achieve the clas...
Claims
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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84
CPCG01N21/84
Inventor 刘怡周炜翔张飞翔方旭东毛欣怡朱博威阮肖镕惠国华张建锋郜园园易晓梅
Owner ZHEJIANG FORESTRY UNIVERSITY
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