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Application of tomato slbzr1l gene

A gene, tomato technology, applied in the application field of tomato SlBZR1L gene in regulating fruit firmness

Active Publication Date: 2021-05-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current studies only focus on the function of BES1 in Arabidopsis against stress

Method used

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  • Application of tomato slbzr1l gene
  • Application of tomato slbzr1l gene
  • Application of tomato slbzr1l gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] The present invention is further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited thereto:

[0019] 1. Obtain the full-length sequence of the tomato SlBZR1L gene:

[0020] Use Primer Premier 6.0 to design whole-gene amplification primers, take the wild-type tomato Ailsa Craig (AC) leaf cDNA planted in the greenhouse of Zijingang Campus of Zhejiang University as a template (the extraction method of cDNA is conventional technology, for example, refer to CN104561025A), and design specific Primers SlBZR1L-F and SlBZR1L-R were used to amplify the SlBZR1L fragment by PCR with PrimerSTAR high-fidelity enzyme.

[0021] The primer sequence is: SlBZR1L-F: 5'-ATGTGGGAAGGTGGAGGGTTGC-3'

[0022] SlBZR1L-R: 5'-CATCCGAGCAGTCCCACTTCCGA-3'

[0023] PCR amplification reaction system: 2xPrimerSTAR buffer 25ul, dNTP Mixture 5ul, PrimerSTARDNA polymerase 1ul, ddH 2 O 16ul, 1ul of cDNA, 1ul of upstream and downstream pri...

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Abstract

The invention belongs to the field of biotechnology, and specifically relates to the application of the tomato SlBZR1L gene in regulating fruit firmness. The nucleotide sequence of the SlBZR1L gene is as described in SEQ ID NO: 1, and the SlBZR1L gene plays a negative regulatory role in improving fruit firmness.

Description

technical field [0001] The invention belongs to the field of biotechnology; in particular, it relates to the application of tomato S1BZR1L gene in regulating fruit firmness. Background technique [0002] Tomato is native to South America and is one of the most widely cultivated vegetable crops in the world. my country's tomato planting area and output rank among the top in the world. With the increase of tomato yield, the loss of shelf life after harvest of tomato has become an important problem restricting the development of tomato industry. How to improve the quality of tomato fruit so as to prolong the storage time of tomato is attracting more and more attention from tomato sellers and scientific researchers. The firmness of tomato fruit is the primary factor affecting the shelf life of tomato fruit. Improving fruit firmness can significantly prolong the shelf life of tomato fruit; prolonging the shelf life of fruit can reduce postharvest losses and effectively avoid the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/08A01H6/82
CPCC07K14/415C12N15/8261
Inventor 汪俏梅刘浩然刘丽红邵志勇孟凡亮梁冬怡
Owner ZHEJIANG UNIV
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