Field planting method for efficiently mating maize diallel crossing combinations
A technology of double row hybridization and planting method, which is applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of pollen hybrid field listing and harvesting, low preparation efficiency, and error-prone, and reduce pollen hybridization. probability, less error-prone, and the effect of reducing errors
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Embodiment 1
[0031] In the winter of 2016, a 13×8 incomplete diallel hybrid was prepared in Xishuangbanna, Yunnan ( Figure 4 ).
[0032] 1) Female parent group: 13 maize inbred lines newly selected by Chongqing Academy of Agricultural Sciences (number: 16Y118-16Y130); male parent group: backbone lines Yu 1193, Yu 213, YA8201, LH8012 and 4 Chongqing Academy of Agricultural Sciences Newly selected maize inbred line (number: 16Y114-16Y117).
[0033] 2) Take the respective inbred lines in the male parent group, plant 1 row for each male parent inbred line, a total of 8 rows, with a row length of 7 meters, and plant 16 litters in two phases, the first phase is the same as the female parent inbred line Plant 8 litters, and plant the second period 3 days later.
[0034] 3) Take the respective inbred lines in the female parent group, and plant 1 column for each female parent inbred line, making it 13 female parent columns, and the first row and the first row of male parent selfing of all female p...
Embodiment 2
[0039] In 2017, this method was used to assemble a 16×8 diallel hybrid combination, and the specific experimental steps were the same as in Example 1.
Embodiment 3
[0041] In 2018, this method was used to assemble a 18×8 diallel hybrid combination, and the specific experimental steps were the same as in Example 1.
[0042] In conclusion, using this method for the preparation of diallel hybrid combinations, the advantages of simplicity, time saving, and difficulty in mixing pollen are more obvious in the actual operation.
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