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Composition for detecting natural antibody for lung cancer, kit and method

A technology of natural antibodies and compositions, applied in the field of immunology, can solve the problem of high risk of tumors

Active Publication Date: 2019-08-27
QINGDAO HAILANSHEN BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Based on this, it is speculated that the risk of developing tumors in people with natural antibody deficiency may be significantly higher than that in normal people

Method used

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  • Composition for detecting natural antibody for lung cancer, kit and method
  • Composition for detecting natural antibody for lung cancer, kit and method
  • Composition for detecting natural antibody for lung cancer, kit and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0064] 1. Sample collection

[0065] 213 plasma samples from patients with non-small cell lung cancer (squamous lung cancer and glandular lung cancer) before surgery and 200 healthy people were collected for detection of plasma NALCIgG levels. All plasma samples were stored at -80°C before operation, and it was verified that the storage time did not exceed two years, and there was no repeated freezing and thawing (no more than 3 times).

[0066] 2. Sample detection

[0067] The plasma samples were thawed at 4°C. The three peptide antigens used in this experiment (see Table 4) were synthesized by SEVERNBIOTECH Co., Ltd., UK, with a purity of 95%. The specific steps were as follows:

[0068] (1) Before operation, each antigen was dissolved in 67% acetic acid into a 5.7 mg / ml stock solution, then mixed in equal volumes and stored in a -20°C refrigerator.

[0069] (2) At the beginning of the operation, firstly, the three kinds of antigen mixtures were diluted to 30 μg / ml with th...

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Abstract

The invention relates to a composition for detecting a natural antibody for lung cancer (NALC) and a kit prepared from the composition, a method for detecting the concentration of the NALC in a sampleby using the composition or the kit, and an application of the composition or the kit in the NALC detection of a sample. According to the method disclosed by the invention, three linear antigen polypeptides which are completely complementary with the target NALC are utilized to realize qualitative and quantitative detection of the NALC in a sample, and can be used for predicting the incidence risk of the lung cancer and early diagnosis of the lung cancer.

Description

technical field [0001] The invention belongs to the technical field of immunology, relates to three kinds of peptide antigens, and can be applied to the preparation of an enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA) antibody detection kit for detecting anti-lung cancer natural antibodies (Natural Antibodies) in human blood. Antibody for lung cancer, NALC) concentration. Background technique [0002] Malignant tumors are one of the most important killers threatening the health of the whole human being. Due to the continuous existence of environmental pollution such as smog and smog, the incidence of major cancers in the Chinese population is increasing year by year. According to statistics, the average annual incidence of cancer in my country is as high as 3 / 1000 or more. Early tumor treatment methods, such as surgical resection or radiation therapy, have been developed relatively maturely, which can significantly prolong the survival time of...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/6854G01N33/57423G01N33/57488G01N2333/715G01N2333/71G01N2333/4725
Inventor 韩传伟张萱徐宏海
Owner QINGDAO HAILANSHEN BIOTECH
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