A kind of liquid artificial feed of Neoseiid mite and preparation method thereof

A technology of Neoseius pasteurii and artificial feed, applied in animal feed, animal feed, animal husbandry, etc., can solve the problems of difficult separation and counting of predatory mites and prey, difficult harvesting period of predatory mites, and large space occupation, etc., to achieve The beneficial effect is remarkable, the cost is low, and the effect of easy operation

Active Publication Date: 2021-07-20
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the above-mentioned prey is used for feeding, there are problems such as large space occupation, difficulty in separating and counting predatory mites and prey, and difficulty in controlling the harvesting period of predatory mites.
In addition, rearing predatory mites with alternative prey acarina poses health risks to production workers and sometimes causes human allergies during release (Bolckmans and Van Houten, 2006; Fernandez-Caldaset al, 2007)

Method used

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  • A kind of liquid artificial feed of Neoseiid mite and preparation method thereof
  • A kind of liquid artificial feed of Neoseiid mite and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The artificial feed of the present embodiment is made up of the material of following weight:

[0031] Bombyx mori chrysalis hemolymph 12g, sucrose 2.4g, honey 2.4g, peptone 2.4g, yeast extract 2.4g, fresh egg yolk 4.8g and water 33.6mL.

[0032] Preparation:

[0033] Rinse the fresh silkworm chrysalis with clean water repeatedly, then take out the silkworm chrysalis, place it in warm water at 60°C for 10 minutes (to prevent blackening), put it in a petri dish, disinfect it with 75% alcohol, rinse it with clean water, and then dry it with filter paper. Cut off the pupal tail, collect the pupal hemolymph and place it in a -20°C refrigerator for later use; shell fresh eggs, collect the egg yolk and put it in a beaker for later use; according to the weight of each material in the examples, weigh each component with a balance and mix them evenly; The uniformly mixed solution is centrifuged, and the supernatant is taken to obtain the liquid artificial feed. The artificial ...

Embodiment 2

[0035] The artificial feed of the present embodiment is made up of the material of following weight:

[0036] Silkworm chrysalis hemolymph 12.8g, shelled artemia eggs 3.2g, sucrose 2.4g, honey 2.4g, peptone 2.4g, yeast extract 2.4g, fresh egg yolk 4.8g and water 33.6mL.

[0037] Preparation:

[0038] Rinse the fresh silkworm chrysalis with clean water repeatedly, then take out the silkworm chrysalis, place it in warm water at 60°C for 10 minutes (to prevent blackening), put it in a petri dish, disinfect it with 75% alcohol, rinse it with clean water, and then dry it with filter paper. Cut off the pupal tail, collect the pupal hemolymph and place it in a -20°C refrigerator for later use; grind the shelled artemia eggs into powder in a mortar for later use; shell fresh eggs, collect the yolk and put them in a beaker for later use; according to the examples The weight of each substance is weighed by a balance and mixed uniformly; the uniformly mixed solution is centrifuged, and ...

Embodiment 3

[0040] The artificial feed of the present embodiment is made up of the material of following weight:

[0041] 12.8g of rice moth eggs, 3.2g of shelled artemia eggs, 2.4g of sucrose, 2.4g of honey, 2.4g of peptone, 2.4g of yeast extract, 4.8g of fresh egg yolk and 33.6mL of water.

[0042] Preparation:

[0043] Pick out the impurities from the fresh rice moth eggs, grind them into a homogenate with a mortar, put them in a beaker, seal them with plastic wrap and put them in a refrigerator at 4°C for later use; grind the shelled Artemia eggs into powder for later use; shell fresh eggs , collect the egg yolk and place it in a beaker for later use; according to the weight of each material in the embodiment, weigh each component with a balance and mix them uniformly; centrifuge the uniformly mixed solution, and take the supernatant to obtain a liquid artificial feed. The artificial feed was stored in a centrifuge tube and stored in a -20°C refrigerator for later use.

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Abstract

The invention discloses a liquid artificial feed for Neoseius pasterii and a preparation method thereof. The formula of the liquid artificial feed of the invention mainly includes rice moth eggs, shelled artemia eggs, sucrose, honey, peptone, yeast extract, fresh egg yolk and water. The preparation method is simple, the raw materials are commercialized, and the source is wide and easy to obtain. The artificial feed provided by the invention can meet the nutritional requirements of Neoseiius pasteurii, has good breeding effect, and can be used for large-scale breeding of Neoseiius pastereii.

Description

technical field [0001] The invention belongs to the field of natural enemy predatory mite breeding, and in particular relates to an artificial feed for Neoseiusius pasterius and a preparation method thereof. Background technique [0002] For a long time, due to the abuse and misuse of chemical pesticides, many small sap-sucking pests such as spider mites, thrips, whitefly, and aphids have been rampant on various crops, and chemical control has become more and more difficult. In contrast, predatory mites can effectively control the above-mentioned types of pest mites due to their small size, rapid growth, large reproductive capacity, strong predation ability, and occupying similar ecological niches with pests (Xu Xuenong, 2013) . [0003] Neoseius pasteurii Neoseiulus barkeri , belonging to Acari, Parasitiforme, Gamasida, Phytoseiidae, and Neoseiidae Neoseiulus . It belongs to the species of polyphagous predatory mite, and its natural food includes spider mites, tarsal m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/20A23K10/22A23K10/16A23K20/147A23K20/163A23K50/00
CPCA23K10/16A23K10/20A23K10/22A23K20/147A23K20/163A23K50/00
Inventor 刘怀李亚迎周海伟
Owner SOUTHWEST UNIV
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