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Method for preparing platelets using reciprocating stirring device

A technology of reciprocating motion and manufacturing method, applied in mixers with rotating stirring devices, transportation and packaging, chemical instruments and methods, etc., can solve problems such as labor-intensive, unsuitable for mass cultivation, etc., to improve production efficiency and suppress abnormalities Platelets, the effect of suppressing the deterioration reaction

Pending Publication Date: 2018-08-31
MEGAKARYON CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the static culture system is very labor-intensive and not suitable for mass culture

Method used

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  • Method for preparing platelets using reciprocating stirring device
  • Method for preparing platelets using reciprocating stirring device
  • Method for preparing platelets using reciprocating stirring device

Examples

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Embodiment 1

[0073] Hereinafter, the present invention will be specifically described based on examples, but the present invention is not limited thereto. Those skilled in the art can change the present invention into various forms without departing from the gist of the present invention, and such changes are also included in the scope of the present invention.

[0074] 1. Fabrication of Immortalized Megakaryocytes

[0075] 1-1. Preparation of hematopoietic precursor cells from iPS cells

[0076] According to the method described in Takayama N., et al. J Exp Med. 2817-2830 (2010), transfer from human iPS cells (TKDN SeV2: iPS cells derived from human fetal skin fibroblasts established using Sendai virus) to Differentiation culture of blood cells. That is, human ES / iPS cell colonies were co-cultured with C3H10T1 / 2 feeder cells in the presence of 20 ng / mL of VEGF (R&DSYSTEMS) for 14 days to produce hematopoietic progenitor cells (HPCs). At 20% O 2 , 5% CO 2 (Unless otherwise specified, ...

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Abstract

The invention provides a method for preparing platelets, the method comprising a step for culturing megakaryocytic cells in a culture solution within a culture vessel, wherein, at said culturing step,the culture solution is stirred by a stirring blade moving reciprocally.

Description

technical field [0001] The present invention relates to a method for producing platelets using a stirring device in which stirring blades reciprocate. Background technique [0002] For the purpose of treating and preventing symptoms thereof, platelet preparations are administered to patients showing a tendency to bleed following massive bleeding at the time of surgery, at the time of injury, or thrombocytopenia after treatment with anticancer agents. Currently, the manufacture of platelet preparations relies on blood donations from healthy volunteers. However, in Japan, the number of blood donors has decreased due to the demographic structure, and it is estimated that there will be a shortage of about 1 million blood donations in 2027. Therefore, as one of the objects of the technical field of the present invention, stable supply of platelets can be mentioned. [0003] In addition, since the existing platelet preparations have a high risk of bacterial contamination, seriou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078A61K35/19A61P7/00C12N5/10A61K35/545C12M3/00C12N15/09B01F27/91
CPCC12N15/09A61K35/19C12M27/02A61K35/14C12N5/0644C12N2500/38C12N2500/25C12N2501/165C12N2501/145C12N2501/125C12N2501/727C12N2501/734C12N2510/04C12N2506/45C12N2527/00B01F27/112B01F27/91A61K35/545C12M3/08C12N5/16C12M3/00C12N5/10C12M27/00C12M21/00C12M29/00C12M41/48C12M1/02C12N15/85C12N2015/8572
Inventor 重盛智大岡本阳己加藤好一
Owner MEGAKARYON CORP
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