Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

major histocompatibility complex single nucleotide polymorphism

一种单核苷酸多态、寡核苷酸的技术，应用在仪器、生物化学设备和方法、分析材料等方向，能够解决成功结果低等问题

Active Publication Date: 2021-06-01
ILLUMINA INC
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, even with an HLA match, the likelihood of a successful outcome is usually still lower than that of haploidentical siblings

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • major histocompatibility complex single nucleotide polymorphism
  • major histocompatibility complex single nucleotide polymorphism
  • major histocompatibility complex single nucleotide polymorphism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0169] Example 1. Ancestral haplotype specificity of SNPs in the C4 gene.

[0170] Sequence-specific primers (SSP) were used to detect 8.1 ancestral haplotype (AH)-specific SNP pairs in the complement C4 gene ( figure 2 ). Sample 1 had 8.1 AH of alpha, beta and delta block markers and tested positive for gamma block SNP markers. Samples 2, 3 and 4 did not have any markers for 8.1 AH and the SSP test for the gamma block SNP marker was negative.

[0171] image 3 The table of shows that many samples with the same C4 allotype have different SNP profiles. Some SNPs are found in multiple samples, while some appear to be unique to a sample (ancestral haplotype). The data suggest that the SNP profile is a marker for the gamma block of the MHC ancestral haplotype and not the allotype of the sample. #8 and #15 gamma-type assays are control SSP assays for C4A and C4B and are not listed.

Embodiment 2

[0172] Example 2. Exemplary PCR Typing Kit

[0173] The inventors of the present invention have developed a PCT assay kit for matching patients and donors to reduce the risk of severe acute graft-versus-host disease. The PCR kit contains PCR buffer, dNTP, MgCl 2and 26 mixes of sequence-specific primers and a single vial of DNA polymerase. Each mix used to amplify the C4 gene SNP is provided in a single bottle of 880 μl. The SNPs to be amplified in the assay are A13189, T14952, G12749, A13950-A14483, G12071, A11483, T9763, A12152, A14831, T14757, C16954, T9881, C9796, A12568-A128389, T19528 , A11437, T14563, A20170, T17316, C10676, G15108, T10309 and / or one or more of C13193. The assay kit further comprises a control mixture for amplifying internal fragments of the C4A and C4B genes.

[0174] Preferably, the DNA sample to be tested in the assay kit is high-molecular-weight human genomic DNA at a concentration range of 20-100 ng / μl in Tris / EDTA buffer, and the OD 260 / 280 >1...

Embodiment 3

[0192] Example 3. Matching patients for gamma type (SNP).

[0193] Patients and donors were typed using PCR-SSP assays for SNP groups. A patient / donor pair was defined as a mismatch if either the donor or the patient was positive for at least one SNP from the panel of 26 SNPs and the corresponding donor / patient was not ( Figure 4 and Figure 5 ). Figure 4 showed that gamma-type mismatches lead to an increased risk of severe aGVHD. Matched (M) and mismatched (MM) patients (x) for gamma type, and whether the patient was diagnosed with acute GVHD and acute GVHD grade. Figure 5 It was shown that gamma-type matching leads to an increased chance of long-term survival.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the identification of single nucleotide polymorphisms (SNPs) in the gamma genomic block of the central region of the major histocompatibility complex (MHC), which can be used to match transplant donors and recipients and determine disease susceptibility. emotional.

Description

technical field [0001] The present invention relates to the identification of single nucleotide polymorphisms (SNPs) in the gamma genomic block of the central region of the major histocompatibility complex (MHC), which can be used to match transplant donors and recipients and determine disease susceptibility. emotional. Background technique [0002] The major histocompatibility complex (MHC) is a gene-dense region of approximately 4 Mb on the short arm of chromosome 6. The MHC contains many immune response genes, including those encoding human leukocyte antigens (HLA). MHC also contains many genes involved in immune response and inflammatory response, and is associated with various autoimmune and inflammatory diseases. [0003] The MHC is usually inherited as a complete block; however, specific regions of the MHC have been shown to recombine at a frequency of about 1 / 100 meiosis. Between these recombination hotspots are genomic blocks of DNA up to a few hundred kb in leng...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12Q1/6858
CPCC12Q2600/156C12Q1/6881C12Q1/6883C12Q2600/106C12Q2600/158C12Q2600/16G01N27/447
Inventor D·C·塞伊尔H·M·霍干K·梅尔考利亚
Owner ILLUMINA INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com