A kind of kelp heavy ion radiation mutation breeding method

Abstract

The invention discloses a novel kelp heavy ion radiation mutation breeding method. The novel kelp heavy ion radiation mutation breeding method comprises the following steps: dispersing and filtering a kelp gametophyte cloning cell system to each gametophyte cell segment composed of 1-3 gametophyte cells; radiating by using 12C heavy ions; breeding a sporophore by using a kelp gametophyte cloning breeding technology; after the sporophore is ripen, carrying out phenotype and quality character determination; carrying out DNA (Deoxyribonucleic Acid) variation detection on candidate plants with a large forward direction variation range; obviously improving phenotype and quality characters and taking the plants with obviously-varied DNA structures as breeding materials for breeding new varieties. The novel kelp heavy ion radiation mutation breeding method has the advantages that a lot of variation can be generated by radiating and inducing kelp gametophytes by the heavy ions; the variant plants can be obtained in shorter time and a new gene pool related with the good property is preserved.

Description

technical field [0001] The invention belongs to the technical field of marine organisms and discloses a kelp heavy ion radiation mutation breeding method. Background technique [0002] Kelp is the large seaweed with the widest breeding area and the greatest economic value in my country. The genetic improvement and breeding of kelp in my country has been carried out in the late 1950s, and breakthroughs have been made in the construction of gametophyte cloning cell lines and hybrid breeding technology. However, since there is no natural distribution of kelp in my country, the available germplasm resources are very limited. Therefore, the differences in traits among different varieties (lines) are not obvious, and the effect of variety improvement is still very limited. Radiation breeding has the advantages of high mutation rate and short breeding cycle. It can induce a large number of new gene sources, greatly enrich the raw materials in breeding work, and thus greatly change ...

AI Technical Summary

Problems solved by technology

The genetic improvement and breeding of kelp in my country has been carried out in the late 1950s, and breakthroughs have been made in the construction of gametophyte cloning cell lines and hybrid breeding technology. However, since there is no natural distribution of kelp in my country, the available germplasm resources are very limited Therefore, there is no obvious difference in traits among different varieties (lines), and the effect of variety improvement is still very limited.

At present, there are no reports involving the use of heavy ion radiation for seaweed breeding