Processing method of viable clostridium butyricum preparation and culture medium composition

Abstract

The invention discloses a processing method of a viable clostridium butyricum preparation and a culture medium composition. The processing method comprises the following steps: carrying out activation and enlarge cultivation on strains; culturing the strains by utilizing a seeding tank; carrying out fermentation cultivation by utilizing a fermentation tank, wherein a fermentation culture medium comprises the following components: corn flour, peptone, beef extract powder, yeast extracts, dipotassium phosphate, monopotassium phosphate, calcium chloride, magnesium sulfate, manganese sulfate, ferrous sulfate, sodium acetate and cysteine salt. According to the processing method, by optimizing the culture medium and a processing process, the fermentation cultivation is short, and the maturing rate of spores is high; furthermore, a thallus collection method is further optimized, so that the production process is greatly simplified, the energy consumption can be substantially reduced, and the yield of active thalli can be improved.

Description

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AI Technical Summary

Benefits of technology

This patented technology improves how clostridia are grown by reducing their growth cycle length while increasing cell size during culturing. It achieves this through optimization with specific media compositions or processes that shorten up the overall duration of each step. Additionally, it allows for more efficient collection of bacterial colonies from these solutions compared to traditional methods like incubating them at room temperature beforehand. Overall, this new approach makes producing high-quality crops faster and easier than current techniques.

Problems solved by technology

The technical problem addressed by this patented method for producing clostridiocolve (C.) Bacteria that are resistant against various factors such as pH value, temperature, oxygen concentration, nutrients, enzyme activity, antibodies, growth conditions, and others during production processes remains unexplored despite their promise being explanted from use in medical applications like treating gastrointestinal disorders caused by harmful substances released into bloodstream fluid due to ingestion thereof.