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A culture method for improving carbon fixation and oil production capacity of microalgae

A cultivation method and technology of microalgae, applied in the cultivation field of improving microalgae's carbon fixation and oil production capacity, can solve the problems of inhibiting microalgae growth, achieve the effect of improving carbon fixation and oil production capacity, and increasing tolerance

Active Publication Date: 2015-11-11
博露(厦门)生物股份有限公司
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Problems solved by technology

However, many studies have shown that the continuous supply of high-concentration carbon dioxide inhibits the growth of microalgae

Method used

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  • A culture method for improving carbon fixation and oil production capacity of microalgae
  • A culture method for improving carbon fixation and oil production capacity of microalgae

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Embodiment 1

[0009] Example 1. This example improves Pseudochlorococcum ( N.salina ) The specific steps of the cultivation method of carbon sequestration and oil production capacity are as follows:

[0010] 1. Cultivation of microalgae: Pseudochlorococcum N.salina Inoculated into three Erlenmeyer flasks containing f / 2 medium, with an initial pH of 8 and a temperature of 23-27 o C, light 65~95μmolm -2 s -1 . Nutrient-rich f / 2 medium, each liter contains: 33.6g artificial sea salt, 0.075g NaNO 3 , 0.00565gNaH 2 PO 4 ·2H 2 O, 1ml trace elements (T) and 1ml vitamins. Among them, each liter of trace element T contains: 4.16gNa 2 EDTA, 3.15gFeCl 3 ·6H 2 O, 0.18gMnCl 2 ·4H 2 O, 10mgCoCl 2 ·6H 2 O, 10mgCuSO 4 ·5H 2 O, 22mgZnSO 4 ·7H 2 O, 6mgNa 2 MoO 4 ·2H 2 O. Each liter of vitamins contains: 100mg vitamin B1, 0.5mg vitamin B12 and 0.5mg vitamin H.

[0011] 2. The supply of carbon dioxide: to three pseudochlorococcum N.salina Air with different concentrations of carbon dioxide is introduced into the c...

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Abstract

The invention discloses a culture method for improving microalgae carbon fixing and oil producing capacity, and relates to the technical field of algae organism culture. According to different demands of microalgae on inorganic carbon sources in different stages (lag phase, the incipient stage of logarithmic phase and the middle and later periods of the logarithmic phase) of a growth cycle, the culture method gradually increases the supply of carbon dioxide, not only greatly improves the growth rate of the microalgae and the tolerance of carbon dioxide concentration, but also greatly improves the microalgae carbon fixing and oil producing capacity. The carbon fixing amount in of the microalgae cultured in a progressive-increase manner is respectively increased by 15 times and 32% compared with that of the microalgae cultured in continuous high-concentration carbon dioxide of 18-22% and continuous air. The oil contents of the microalgae cultured in the continuous high-concentration carbon dioxide of 18-22%, in the continuous air and in the progressive-increase manner are respectively 29%, 24% and 47%. The oil producing amount of the microalgae cultured in the progressive-increase manner is respectively increased by 7.7 times and 1.6 times compared with that of the microalgae cultured in the continuous high-concentration carbon dioxide of 18-22% and the continuous air.

Description

Technical field [0001] The invention relates to a cultivation technique of algae organisms, in particular to a cultivation method for improving the carbon fixation and oil production capacity of microalgae. Background technique [0002] Greenhouse gases, including carbon dioxide, cause global warming and climate change. The control of greenhouse gases, especially carbon dioxide emissions, has become one of the common global issues. With the rapid economic development of China, carbon dioxide emissions are also rising rapidly. At present, the total emissions have reached the first place in the world. As one of the signatories of the "Kyoto Protocol", although China is not obligated to reduce emissions for developing countries, the control of carbon dioxide emissions is an unavoidable responsibility. The vast majority of carbon dioxide emissions come from the burning of traditional fossil energy sources, and this part accounts for more than three-quarters of human carbon dioxide ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 陈意民
Owner 博露(厦门)生物股份有限公司
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