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Application of chnrra protein or its encoding gene in regulating plant flowering time

A technology that encodes genes and flowering time, applied in the biological field, can solve problems such as consumption, and achieve the effect of great application potential

Active Publication Date: 2015-10-28
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of cultivating ornamental flowers, artificial photoperiod, artificial temperature control and spraying of plant hormones are usually used to regulate flowering, which consumes a lot of space, energy and labor costs.

Method used

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  • Application of chnrra protein or its encoding gene in regulating plant flowering time
  • Application of chnrra protein or its encoding gene in regulating plant flowering time
  • Application of chnrra protein or its encoding gene in regulating plant flowering time

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Experimental program
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Embodiment 1

[0049] Embodiment 1, the application of ChNRRa gene in regulating the flowering time of chrysanthemum

[0050] 1. Construction of overexpression vector and RNA interference vector

[0051] 1. Obtaining the cDNA fragment of ChNRRa gene

[0052] According to the conserved nucleotide and deduced amino acid sequences of rice ChNRRa and its homologous genes, four degenerate primers were designed: forward JuS1, JuS2; reverse JuR1, JuR2 (Table 1). Using the TRIZOL (Invitrogen Cat.No.15596-018) method, total RNA was extracted from the young leaves of Xiahuang (a variety of chrysanthemum), and the total RNA was extracted according to TURBO DNA-free TM After Kit (Applied Biosystems, AM1907) was treated with TURBO DNase, Invitrogen superscript was used TM III first strand synthesis system (Cat.No.18080-051) synthesized cDNA by reverse transcription, and used it as a template for a degenerate PCR reaction. Four primer combinations (a) JuS1+JuR1; (b) JuS1+JuR2; (c) JuS2+JuR2; and (d) ...

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Abstract

The invention discloses application of a ChNRRa protein or a coding gene thereof in adjusting the flowering time of a plant. The amino acid sequence of the ChNRRa protein provided by the invention is represented by sequence 4 in a sequence table. According to results of experiments in the invention, the flowering time of a plant is adjusted by adjusting the expression level of NRR (nutrition response and roof growth) genes (comprising an OsNRRa gene in paddy rice and orthologous genes in other monocotyledons and a ChNRRa gene in chrysanthemum and orthologous genes in other dicotyledons) in the plant.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a ChNRRa protein or its coding gene in regulating plant flowering time. Background technique [0002] Plant flowering is affected by many internal and environmental factors, such as developmental stage, hormones, light and temperature. In the cultivation process of ornamental flowers, artificial photoperiod, artificial temperature control and spraying of plant hormones are usually used to regulate flowering, which consumes a lot of space, energy and labor costs. Contents of the invention [0003] One object of the present invention is to provide the application of ChNRRa protein or its coding gene. [0004] The invention provides the application of ChNRRa protein or its coding gene in regulating plant flowering time; [0005] The amino acid sequence of the ChNRRa protein is sequence 4 in the sequence list. [0006] In the above application, the gene encoding C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/84A01H5/00C12N15/29
Inventor 方荣祥张玉满陈晓英刘青林
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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