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Method for breeding inbred line of purple solanum lycopersicum

An inbred line, tomato technology, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the problems of single genotype food safety and other issues

Active Publication Date: 2014-02-26
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for homozygous inbred purple tomato germplasm resources with a short cultivation period, good agronomic traits and different genotypes in view of the problems of single genotype and edible safety in the existing purple tomato

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: (Breeding of purple tomato CL1-1, CL1-2 inbred lines)

[0025] 1) July-November 2007: The red tomato T07-026 was used as the female parent and the yellow tomato T07-021 was used as the male parent to obtain the F1 generation material 2621, and the fruit color was red;

[0026] 2) December 2007-June 2008: The green tomato CH09-004 was used as the female parent and 2621 was used as the male parent to obtain a three-cross variety 09-004×2621 with red and yellow fruit colors;

[0027] 3) July-November 2008: Using CH09-004 as the reincarnation female parent and 09-004×2621 red plant as the male parent, the backcross generation 09-004×2621BC1 was obtained by crossing;

[0028] 4) December 2008-June 2009: plant 100 backcrossing first-generation plants, and the fruit colors are separated into three types: red, yellow and green, and select green tomato plants with good growth and fruiting performance for self-crossing and saving seeds. Obtain BC1F2 generation see...

Embodiment 2

[0031] Example 2: (Breeding of purple tomato CL2-1, CL2-2 inbred lines)

[0032] 1) December 2006-June 2007: The yellow tomato CH03-056 was used as the female parent, and the red tomato Zheza 203 was used as the male parent. The F1 material number obtained was 5603, and the fruit color was red;

[0033] 2) July-November, 2007: The green tomato CH09-004 was used as the female parent and 5603 was used as the male parent to obtain a three-cross variety 09-004×5603 with red and yellow fruit colors;

[0034] 3) From December 2007 to June 2008: the red plant of 09-004×5603 was used as the female parent, and CH09-004 was used as the reincarnated male parent to obtain the first generation of backcross 09-004×5603BC1;

[0035] 4) July-November 2008: Plant 100 plants of the first generation of backcross 09-004×5603BC1. The fruit colors are separated into three types: red, yellow and green, and the green tomato plants with good growth and good fruit performance are selected for self-bree...

Embodiment 3

[0038] Example 3: (Breeding of purple tomato CL3-1, CL3-2 inbred lines)

[0039] 1) July-November 2007: The red tomato Zheza 204 was used as the female parent, and the yellow tomato CH03-056 was used as the male parent to cross, and the material number of the F1 generation was obtained as 0456, and the fruit color was red;

[0040] 2) From December 2007 to June 2008: Crossing 0456 as the female parent and green tomato CH09-004 as the male parent yielded a three-cross variety 0456×09-004, with red and yellow fruit colors;

[0041] 3) July-November 2008: Crossing the red plant of 0456×09-004 as the female parent and CH09-004 as the recurrent male parent produced a backcross generation 0456×09-004BC1;

[0042] 4) December 2008-June 2009: 100 plants of the first generation of backcross 0456×09-004BC1 were planted, and the fruit colors were separated into three types: red, yellow and green, and a single green tomato plant with good growth and excellent fruiting performance was ...

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PUM

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Abstract

The invention discloses a method for breeding an inbred line of purple solanum lycopersicum, and belongs to the technical field of plant breeding. The method comprises the following steps of: 1) preparing a red F1-generation hybrid by using red and yellow solanum lycopersicum; 2) hybridizing the red F1-generation hybrid which serves as a male parent or a female parent and green solanum lycopersicum to obtain a three-way cross hybrid; 3) back-crossing the red three-way cross hybrid by using the green solanum lycopersicum as a male parent or a female parent to obtain a BC1 generation; 4) selecting a single green plant from a BC1-generation segregation population, and performing inbreeding to obtain a BC1F2-generation seed; 5) selecting the purple solanum lycopersicum from the BC1F2-generation seed for inbreeding seed reservation; and 6) performing inbreeding homozygosis of three to five generations to obtain the inbred line of the purple solanum lycopersicum. A novel breeding means is provided for germplasm resources of the purple solanum lycopersicum, can be used for breeding purple solanum lycopersicum hybrids with distinct advantages, and can be popularized and applied to breeding and production.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for cultivating different types of purple tomato germplasm by using tomatoes with three different fruit colors: red, yellow and green. Background technique [0002] tomato( Solanum lycopersicum ) is one of the most important vegetable crops in the world, with an annual planting area of ​​about 3.989 million hectares and a total output of 108.499 million tons (FAO, 2010). Although tomato has a short history of cultivation in my country, it has become one of the main types of vegetables. Purple tomato is a very unique variety in tomato. In 2000, Carl Jones, a graduate student in Oregon, USA, found that purple tomato not only contains carotene and lycopene that ordinary tomatoes have, but also other nutrients. Anthocyanins, which are not found in tomatoes, have higher nutritional value than ordinary tomatoes. Purple tomato originated from the virgin jungles of Ame...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 杨悦俭阮美颖周国治王荣青叶青静姚祝平李志邈万红建
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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