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Flavanone 3',5'-hydroxylase gene and protein coded by same

A flavanone and hydroxylase technology, applied in the field of genetic engineering, can solve the problem of not finding such genes, etc., and achieve the effect of improving ornamental quality

Inactive Publication Date: 2012-01-04
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no reports of such genes have been found in Aconitum

Method used

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  • Flavanone 3',5'-hydroxylase gene and protein coded by same
  • Flavanone 3',5'-hydroxylase gene and protein coded by same
  • Flavanone 3',5'-hydroxylase gene and protein coded by same

Examples

Experimental program
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Effect test

Embodiment Construction

[0013] 1. Cloning of candidate gene fragments in the flower of Aconitum sichuanensis using merger primers

[0014] In Aconitum aconitum (a well-known public material, Qu Suping et al. "Rapid Propagation of Aconitum Aconitum Seedlings by Tissue Culture" Chinese Wild Plant Resources CHINESE WILD PLANT RESOURCES 2004,23(4):62-63) flowering period, whichever is different developmental period After the flowers were quickly frozen in liquid nitrogen, the total RNA was extracted with TRIZOL (invitogen) according to the reagent instructions. Transcribe it into cDNA, then dilute the cDNA 10 times as a template, and carry out PCR reaction with degenerate primers. The names and sequences of the degenerate primers are as follows:

[0015] W-FH-F: 5'-C(A / T / C / G)CA(A / G)GA(T / C)ATGGT(T / C / G)TT(C / T)G-3'

[0016] W-FH-R: 5’-C(A / T / G)AT(A / T / C / G)GCCCA(A / T / G)AT(A / G)TT

[0017] (A / T / C / G)A-3'

[0018] 0.5 μl each of 10 μM 5’ primer and 3’ primer; 2.5 μl 10× buffer; 2.5 μl 2.5 mM dNTP; 1.5 μl 25 mM m...

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Abstract

The invention provides a flavanone 3',5'-hydroxylase gene and protein coded by the same. The flavanone 3',5'-hydroxylase gene Ac-F3'5'H is from Aconitum carmichaeli Debx, and is named as an Ac-F3'5'H gene, wherein a nucleotide sequence of the Ac-F3'5'H gene is shown as SEQ ID NO. 1, an amino acid sequence coded by the Ac-F3'5'H gene is shown as SEQ ID NO. 2, the flavanone 3',5'-hydroxylase gene Ac-F3'5'H in the Aconitum carmichaeli Debx is reported for the first time, and the expression of the flavanone 3',5'-hydroxylase gene Ac-F3'5'H can promote plants to synthesize delphinium anthocyanins, so that the colors of the plants are expressed as blue. The flavanone 3',5'-hydroxylase gene Ac-F3'5'H is inoculated into other flowers which are deficient in blue color systems, so that flower varieties with blue colors are expected to be cultivated to improve the ornamental performance of the flowers.

Description

technical field [0001] The invention discloses a Aconitum carmichaeli Debx)'s flavanone 3',5'-hydroxylase gene (Ac-F3'5'H) and its encoded protein belong to the technical field of genetic engineering. Background technique [0002] Flower color is one of the most important ornamental and economic traits of ornamental plants. Blue gives people an elegant and dreamy feeling, but currently the world's four major fresh cut flowers lack blue series, so breeding blue flowers has become one of the hotspots in flower color research and improvement in flower breeding for more than ten years. [0003] At present, although conventional breeding methods can breed many new flower and color varieties, it is difficult to break the reproductive isolation between genus and species, and there are great limitations in the utilization of resources, especially wild flower resources. Goals such as blue suits are difficult to achieve. The world's four major cut flowers lack blue varieties, bec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N9/02
Inventor 马璐琳王继华吴丽芳王祥宁张艺萍崔光芬贾文杰
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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