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Application of complete Freund adjuvant (CFA) to preparation of tibia inflammatory pain model

A technology of complete Freund's adjuvant and tibial bone, applied in the model of osteoinflammation pain, complete Freund's adjuvant in the field of preparation of tibial inflammatory pain model, has achieved important academic research value and application prospect

Inactive Publication Date: 2011-05-25
FUDAN UNIV
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  • Application of complete Freund adjuvant (CFA) to preparation of tibia inflammatory pain model
  • Application of complete Freund adjuvant (CFA) to preparation of tibia inflammatory pain model
  • Application of complete Freund adjuvant (CFA) to preparation of tibia inflammatory pain model

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Embodiment 1

[0025] 1. Materials and methods:

[0026] Inflammatory agent: Complete Freund's adjuvant (Complete Freund's adjuvant, CFA), purchased from Sigma-Aldrich China Branch;

[0027] Animals: SD female rats, weighing 140-160 grams, provided by the Shanghai Experimental Animal Center of the Chinese Academy of Sciences;

[0028] Main instruments: von Frey hair (Stoelting, Wood Dale, Illinois, USA); radiant heat pain measuring instrument (Model 390 Paw Stimulator Analgesia Meter, IITC / Life Science Instruments, USA);

[0029] Modeling method:

[0030] Model group: After the rats were anesthetized by intraperitoneal injection of 8% chloral hydrate, the hair on the knee joint was shaved, and the surface skin was disinfected with iodine or 70% alcohol. Along the longitudinal axis of the tibia to the medullary cavity at the distal end of the tibia, insert 5-30ul of CFA and apply iodine for disinfection;

[0031] Control group: The same volume of normal saline was implanted into the medull...

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Abstract

The invention belongs to the technical field of biology, relates to a bone inflammatory pain model, in particular to application of a complete Freund adjuvant (CFA) to the preparation of a tibia inflammatory pain model. Tests on the tibia histopathology and behavioral indicators such as a heat pain threshold, a mechanical pain threshold and the like of the bone inflammatory pain model prepared by the CFA prove that: the inflammation of an experimental model is obvious, and the level of the behavioral indicators is low. A valuable bone inflammatory pain contrast model is provided for the research of bone cancer pain, particularly the mechanism research of cancer pain; a valuable experimental model is provided for the research of pain caused by clinical osteomyelitis; meanwhile, the type of a chronic pain experimental animal model is enriched, and the prepared tibia inflammatory pain model has important academic research value and application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to a model of osteoinflammation pain, in particular to the use of complete Freund's adjuvant (CFA) in preparing the model of tibia inflammatory pain. Background technique [0002] Osteomyelitis is a common disease, and the severe pain caused by repeated stimulation of the periosteum due to inflammation seriously affects the quality of life of patients. However, there are still few reports on the pain caused by bone inflammation. Therefore, it is of great practical significance to establish a stable model of bone inflammation with simple and feasible methods. The establishment of osteoinflammatory pain model will provide a valuable animal model for the study of pain caused by osteomyelitis. [0003] Complete Freund's adjuvant (CFA) is a commonly used inflammatory agent, and the prior art discloses that Complete Freund's adjuvant (CFA) is widely used in the production of chronic pain models su...

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Application Information

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IPC IPC(8): A61K39/29A61K49/00A01K67/027
Inventor 毛应启梁杨长江王彦青
Owner FUDAN UNIV
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