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Peptide vaccine for animal and preparation method thereof

A synthetic peptide vaccine and vaccine technology, applied in the peptide field of foot-and-mouth disease synthetic peptide vaccine, can solve the problems of ineffective protection of animals, influence on the use of new vaccines, poor effect, etc., achieve good application prospects, easy large-scale synthesis, and enhance immunity effect of effect

Inactive Publication Date: 2009-10-28
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes how changes made over years may affect viruses like Foot-and_ mouth Disease (FMD) caused by bacteria called ActAidium parvum or other similar microorganisms found around shopping malls during winter months. By analyzing this change for potential new locations within each infectious cycle, we aim to predict where they will become active again after being exposed to another type of pathogen such as FMD. These candidates could then help create more effective treatments against future outbreaks.

Problems solved by technology

This patents discusses various methods aiming at controlling this serious problem due to the wide range of types of foot diseases called feline calf syndrome viruses (FCSV's). These include FCMV-1, FAVM/MARVD-2, Gulf Coast fever virus, Crimea-Barris Virus, Boviridae, Yellow River Borne Pathogenesis Virus Group 2, Orthobaculosis Vimscae Semenvirus, Toxic Shock Syndrome Virus Lymphoencephalitis Virus(FLUAV) Variant Of All Types Gastroenteropathy Associates (GAPID)). There were previous attempts made towards developing novel vaccination strategies targeting specific pathogens like those mentioned above but none had shown promising outcomes over existing ones.

Method used

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  • Peptide vaccine for animal and preparation method thereof
  • Peptide vaccine for animal and preparation method thereof
  • Peptide vaccine for animal and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The solid-phase synthesis of embodiment 1 foot-and-mouth disease synthetic peptide antigen

[0034] The polypeptide antigen of the present invention can be prepared by a Merrifield solid-phase synthesis method using an automatic polypeptide synthesizer, wherein 9-fluorenylmethoxycarbonyl (Fmoc)-modified amino acids are used, and the solid-phase carrier is Rink Amide MBHA resin. The production process usually includes solid-phase synthesis of polypeptide antigens, cleavage of polypeptides, purification of antigens and sterilized storage.

[0035] 1.1 Solid phase synthesis of polypeptide antigen

[0036] 1.1.1 Preparation of synthetic raw materials

[0037] The sequences of the synthetic polypeptide antigens are respectively shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6.

[0038] According to the sequence of the antigen and the synthesis scale, 1 mmol of the appropriate Fmoc-modified amino acid was prepared and added to the cor...

Embodiment 2

[0064] Example 2. Preparation of synthetic peptide vaccines

[0065] 2.1 Preparation of antigen aqueous phase

[0066] First, weigh the three peptide antigens synthesized according to the above-mentioned Example 1; then, dilute the concentration of the synthesized peptide antigen to 50 μg / ml with sterile water for injection; filter the antigen solution through a filter with a pore size of 0.2 μm, and sterilize .

[0067] 2.2 Preparation of oil phase adjuvant

[0068] Sterilize the oil-phase adjuvant at 121°C for 30 minutes, and set aside.

[0069] 2.3 Emulsion of synthetic peptide vaccine

[0070] Clean the IKA emulsification equipment with 2000ml of sterilized distilled water for 3 times, then put the oil phase into the emulsification tank at 20-28°C according to the volume ratio of oil phase adjuvant and antigen water phase of 1:1, and start the motor to After stirring at a slow speed of 90-150r / m for 2 minutes, slowly add the water-phase antigen at the same time, stir f...

Embodiment 3

[0071] Efficacy test of embodiment 3 synthetic peptide vaccine

[0072] 1. Materials and methods

[0073] 1.1 Synthetic peptide vaccine

[0074] Polypeptide antigens with sequences of SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6 were synthesized according to the above-mentioned examples, and then prepared respectively with The corresponding batch numbers are: ZM62601, ZM626A02, ZM626A03, ZM626A04, ZM626A05, ZM626A06 foot-and-mouth disease synthetic peptide vaccines.

[0075] 1.2 Test animals

[0076] Select 32 healthy pigs of the same breed, 4 months old, weighing about 40Kg, and negative for neutralizing antibodies against foot-and-mouth disease.

[0077] 1.3 Virus seed OR / 80MF 8

[0078] OR / 80MF 6 The virus was collected after being passed through the suckling mice for 2 generations. After passing the poison test, the suckling mice were stored at -20°C for future use.

[0079] 1.4 Test method

[0080] For each group of vaccines, 5 ...

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Abstract

The invention provides an aftosa synthetic peptide vaccine, more specifically to polypeptide for aftosa synthetic peptide vaccine, vaccine containing the polypeptide and a preparation method thereof. The polypeptide has amino acid sequence represented by SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5 and SEQ ID NO.6. By sequencing of recent aftosa strain in China and combination of aftosa vaccine strain sequence, the invention researches variation case of main antigen site, carries out antigen site analysis and forecast assisted by computer, and carries out chemical synthesis to possible antigen site peptide segment. The invention screens polypeptide antigen through a large amount of animal tests, optimizes aftosa virus antigen site according to screening result, effectively combines T cell epitope and B cell epitope, and enhances immunization effect of the polypeptide antigen. The aftosa synthetic peptide can effectively reply antigenic variation of the aftosa virus, has good biological safety, is easy for large scale production, and has better application prospect.

Description

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Claims

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Application Information

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Owner CHINA ANIMAL HUSBANDRY IND
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