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Antigen analogue epi-peptide of para bacillus fowl blood phili and its use

A technology of Haemophilus gallinaceus and mimic epitopes, which is applied in the field of differential diagnosis and preventive medicine for preparing chicken infectious rhinitis, can solve the problems affecting the growth of laying hens and rearing chickens, and achieve good economic benefits and market Foreground, easy-to-manipulate effects

Inactive Publication Date: 2005-03-23
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ensuing problem is: Hpg is a gram-negative bacterium that contains endotoxin, and large-scale inoculation will also affect the egg production of laying hens and the growth of rearing chickens

Method used

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  • Antigen analogue epi-peptide of para bacillus fowl blood phili and its use
  • Antigen analogue epi-peptide of para bacillus fowl blood phili and its use
  • Antigen analogue epi-peptide of para bacillus fowl blood phili and its use

Examples

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Embodiment 1

[0015] Example 1 Application of Mimotope Fusion Peptides in Differential Diagnosis

[0016] 1. Experimental method

[0017] The artificially synthesized oligonucleotide sequence encoding the mimotope peptide CSWYFPSHC (where C represents cystine residues, S represents serine residues, W represents tryptophan residues, Y represents tyrosine residues, and F represents phenylpropanoid residues amino acid residues, P represents proline residues, S represents serine residues, and H represents histidine residues), and the sticky end sequences recognized by EcoR I and HindIII endonucleases are added at both ends. The PGEX-KG expression vector (containing the nucleic acid sequence encoding the GST protein, produced by Promega) was digested with the above-mentioned EcoR I and HindIII endonucleases, and the large fragment was recovered by low-melting point gel, and then the synthetic fragment and the carrier fragment were in a ratio of 5:1 Ligated overnight at 16°C with T4 lig...

Embodiment 2

[0020] Example 2 Display of mimotopes on bacterial flagella and detection of specific antibodies after immunization of mice

[0021] 1. Experimental method

[0022] According to the operation method recommended by the FliTrx system manual of Invitrogen, the artificially synthesized oligonucleotide sequence encoding the mimotope peptide SWYFPSH was inserted between the multiple cloning sites BstX I and Xho I of the FliTrx vector. The mimotope peptide will be displayed in the loop region of bacterial flagellin thioredoxin as CSWYFPSHC. After the correct insertion was verified by enzyme digestion and sequencing, the recombinant plasmid was electroporated into GI826 host bacteria. The recombinant bacteria were cultured in IMC medium at 25°C overnight, and the expression of tryptophan was induced for 6 hours. Twenty male Kunming rats weighing 20±2 g were randomly divided into 4 groups with 5 rats in each group. Experimental group by 10 8 The dosage of each bacteria was injected...

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Abstract

An antigen mimicking epitope peptide of chicken parahemophilus has its amino acid sequence: CSWYFPX1X2C. It can be used to prepare the diagnosing reagent and vaccine of chicken's infectious rhinitis.

Description

technical field [0001] The present invention relates to short peptides simulating antigenic epitopes of Haemophilus paragallinarum, in particular to a group of short peptides capable of simulating the serotype epitopes of Haemophilus paragallinarum type C, and the use of the group of short peptides in preparing chicken infectious Differential diagnosis of rhinitis and its application in preventive medicine. technical background [0002] Haemophilus paragallinarum (Hpg) is a pathogenic bacterium that causes infectious rhinitis in chickens. It can be divided into three serotypes, A, B, and C, and types A and C are the main pathogenic bacteria. Avian infectious rhinitis is an acute respiratory disease with clinical symptoms described as fowl plague, contagious, or infectious catarrh and uncomplicated rhinitis in early literature. Because it is contagious and mainly affects the nasal passages, it is named infectious rhinitis. Its main hazard is to cause a decrease in egg prod...

Claims

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Application Information

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IPC IPC(8): A61K39/102A61P11/04C07K14/195G01N33/68G01N33/96
Inventor 高亚萍柳川孟露露邵宁生辛忠涛张培君
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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