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Application of streptomyces and bacillus in promoting formation of aerobic granular sludge

An aerobic granular sludge and Bacillus technology, which is applied in water/sludge/sewage treatment, chemical instruments and methods, biological water/sewage treatment, etc. Good, well-structured effect

Pending Publication Date: 2022-07-22
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of Penicillium oxalicum can reduce the cultivation time of aerobic granular sludge, the cultivation period is still relatively long, and there is still room for further improvement

Method used

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  • Application of streptomyces and bacillus in promoting formation of aerobic granular sludge
  • Application of streptomyces and bacillus in promoting formation of aerobic granular sludge
  • Application of streptomyces and bacillus in promoting formation of aerobic granular sludge

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Using Streptomyces-Bacillus functional bacterial agent to cultivate aerobic granular sludge in a shaker

[0042] In this example, aerobic granular sludge was cultivated in a shaker by using a triangular flask containing activated sludge, and the bacteria used were Streptomyces with the deposit number of CICC 11030 and Bacillus with the deposit number of CICC 10142.

[0043] S1. Cultivation of Streptomyces: Under sterile conditions, inoculate Streptomyces into Gao's No. 1 medium and place it in a shaker for 4 days under the conditions of 37°C and 250rpm for liquid expansion, and the concentration of colonies is 1×10 7 CFU / mL of Streptomyces granule bacterial solution;

[0044] Each 1 L of Gao's No. 1 medium contains 20 g of soluble starch, 1 g of potassium nitrate, 0.5 g of sodium chloride, 0.5 g of dipotassium hydrogen phosphate, 0.5 g of magnesium sulfate, and 0.01 g of ferrous sulfate, and the pH is 7.2 to 7.4.

[0045] S2. Cultivation of Bacillus: Under st...

Embodiment 2

[0049] Example 2 Using different Streptomyces-Bacillus functional bacterial agents to cultivate aerobic granular sludge in a shaker

[0050] The difference between this example and Example 1 is that the used Streptomyces and Bacillus are different. In this example, the same method as in Example 1 was adopted, and Streptomyces with the deposit number of CICC 11040 and Bacillus with the deposit number of CICC 21298, Streptomyces with the deposit number of CICC 11030 and Bacillus with the deposit number of CICC 21290, and Streptomyces with preservation number CICC 11056 and Bacillus with preservation number CICC 21290 were prepared as Streptomyces-Bacillus functional inoculants, and the prepared functional inoculants were added to the conical flask containing activated sludge , shaking culture to obtain aerobic granular sludge.

[0051] The results showed that adding functional inoculants made of Streptomyces with preservation number of CICC 11040 and Bacillus with preservation ...

Embodiment 3

[0052] Example 3 Using Streptomyces-Bacillus functional bacterial agent to cultivate aerobic granular sludge in a shaker

[0053] In this example, aerobic granular sludge was cultivated in a shaker by using a triangular flask containing activated sludge, and the bacteria used were Streptomyces with the deposit number of CICC 11030 and Bacillus with the deposit number of CICC 10142.

[0054] S1. Cultivation of Streptomyces: under sterile conditions, inoculate Streptomyces into Gao's No. 1 medium and place it in a shaker at 25 °C and 150 rpm for liquid expansion for 2 days, and obtain a colony concentration of 2 × 10 6 CFU / mL of Streptomyces granule bacterial solution;

[0055] S2. Cultivation of Bacillus: Under sterile conditions, inoculate Bacillus into LB liquid medium and place it in a shaker, and expand the liquid for 3 days at 25°C and 150 rpm to obtain a colony concentration of 4 × 10 9 Bacillus bacteria liquid of CFU / mL;

[0056] S3. Cultivation of aerobic granular slu...

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Abstract

The invention discloses application of streptomyces and bacillus in promoting formation of aerobic granular sludge. Streptomyces and bacillus are respectively subjected to amplification culture and then are mixed to prepare the functional microbial inoculum, then the functional microbial inoculum is used for culturing the aerobic granular sludge, and the aerobic granular sludge can be obtained after 4-14 days, so that the time for culturing the aerobic granular sludge can be shortened, and the formed aerobic granular sludge is smooth in surface, compact in structure and high in yield. The functional microbial agent can effectively promote the formation of the aerobic granular sludge, and the formed aerobic granular sludge has good stability, is suitable for rapidly culturing the aerobic granular sludge, and accelerates the starting speed of a membrane bioreactor.

Description

technical field [0001] The invention belongs to the technical field of wastewater biological treatment. More specifically, it relates to the use of Streptomyces and Bacillus in promoting the formation of aerobic granular sludge. Background technique [0002] The use of activated sludge to treat and purify wastewater is the most widely used method in the current sewage treatment field. Among them, aerobic granular sludge (AGS) refers to a granular activated sludge formed by self-aggregation of microorganisms under aerobic conditions. Compared with the traditional activated sludge method, aerobic granular sludge has the advantages of low overall energy consumption, low civil construction cost and good effluent quality. Compared with flocculent activated sludge, aerobic granular sludge has higher microbial content, stronger activity and better settling performance. Compared with anaerobic granular sludge, the formation period of aerobic granular sludge is short, the cultivat...

Claims

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Application Information

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IPC IPC(8): C02F3/12
CPCC02F3/12C02F3/1268C02F2203/004C02F2209/06C02F2209/44C02F2305/06
Inventor 汤兵许悦张焕龙宾丽英
Owner GUANGDONG UNIV OF TECH
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