Attractant for schizophrassica juncea, lure and preparation method of lure for schizophrassica juncea
An attractant and lure core technology, which can be applied in the directions of attracting pests, botanical equipment and methods, biocides, etc., can solve the problem of no attractant, etc., and achieve good attracting effect, high efficiency, Environmentally friendly effect
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[0042] The present invention also provides a method for preparing the lure described in the above scheme, comprising the following steps:
[0043] The attractant is dissolved in a solvent to obtain an attractant solution, and the carrier is used to adsorb the attractant solution to obtain the lure core of the firefly beetle.
[0044] The present invention has no special requirements on the preparation method of the attractant solution, as long as hexanal, cis-3-hexenal and cis-3-hexenol are dissolved in a solvent according to the ratio described in the above scheme.
[0045] The present invention has no special requirements on the adsorption method, and the carrier can naturally adsorb the attractant solution. In a specific embodiment of the present invention, it is preferable to put 10 blank rubber tips into a conical flask as a group, and add 250 μL of attractant solution to the conical flask, and stir with a glass rod while dripping to make the rubber tip evenly adsorbed. ...
Embodiment 1
[0048] (1) GC-EAD analysis of volatiles in the host of Phyllostachys tarsus
[0049] The headspace adsorption method was used to extract the volatiles from the aboveground part of the host plant, Glycyrrhiza uralensis, and to detect the GC-EAD reaction of the antennae of the beetle, and the results were as follows figure 1 shown, according to figure 1 It can be seen that the antennae have electrophysiological responses to 9 separated components, which appeared at 7.17min, 8.20min, 9.97min, 12.15min, 13.20min, 14.00min, 14.35min, 15.53min and 15.98min, and appeared in multiple can be repeated on each antennae.
[0050](2) GC-MS identification of licorice tarsus volatiles
[0051] GC-MS analysis of the volatile extracts from the aerial parts of the host Glycyrrhiza uralensis showed the following results: figure 2 as shown, figure 2 It shows that 9 kinds of separated components were detected successively at 6.73min, 8.44min, 10.95min, 14.91min, 16.74min, 17.81min, 18.57min,...
Embodiment 2
[0063] According to the mass ratio of hexanal, cis-3-hexenal and cis-3-hexenol as the ratio of 10.80:18.78:70.42, the attractant was prepared, and the attractant was dissolved in dichloromethane to obtain a concentration of 100 μg / μL of lure, take the blank rubber-tipped lure out of the package, put 10 pieces into 100ml conical flasks, add 250 μL of lure to each conical flask, and stir with a glass rod while dripping to make the absorption even. Lure cores loaded with 2500ug of active ingredients are sealed and packaged with 10 pieces / bag of aluminum film, and stored at 4°C.
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