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TLC identification method of Xiaobupleurum preparation

A thin-layer identification and Bupleurum-based technology, applied in instruments, measuring devices, scientific instruments, etc., to achieve the effect of reducing usage, simplifying experimental methods, and optimizing thin-layer identification methods

Active Publication Date: 2022-04-08
广东志道医药科技有限公司
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  • Description
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  • Application Information

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Problems solved by technology

[0006] However, no researchers have successfully applied the solid-phase extraction method to the identification of Xiaobupleurum species

Method used

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  • TLC identification method of Xiaobupleurum preparation
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  • TLC identification method of Xiaobupleurum preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] A thin-layer identification method for Xiaobupleurum preparation, comprising the following steps:

[0072] 1. Preparation of the test article

[0073] Take 1g of Xiaobupleurum granules for test, add 10ml of water and shake to dissolve, then add to C18 solid phase extraction cartridge (C18 solid phase extraction cartridge is pre-activated with 5ml of methanol, and then equilibrated with 10ml of water), add 25ml of water Elution, discard the eluent, then use volume percentage concentration 20% ethanol aqueous solution (hereinafter referred to as 20% ethanol), 30% ethanol aqueous solution (hereinafter referred to as 30% ethanol) and 95% ethanol aqueous solution (hereinafter referred to as 95% ethanol) respectively ) each 25ml elution, collect three kinds of eluents respectively, after water bath evaporates to dryness, residue is dissolved with 1ml ethanol respectively, promptly needs testing solution, need testing solution 2 and need testing solution, for subsequent use. ...

Embodiment 2

[0104] In this embodiment, each condition in the identification method of embodiment 1 is screened and investigated.

[0105] 1. Investigation of elution conditions

[0106] Take a small Bupleurum granule sample (batch number: 20200115), dissolve it in water, add two activated C18 solid-phase extraction cartridges (Anpu), and elute with different concentrations of ethanol and methanol, and collect all eluents. Spot on the same GF254 thin-layer plate, then expand, check with the method in embodiment 1, chromatogram sees figure 2 .

[0107] The bands in the figure are numbered as follows:

[0108] 1. Water elution; 2. 10% methanol elution; 3. 20% methanol elution; 4. 30% methanol elution;

[0109] 5. 40% methanol elution; 6. 100% methanol elution; 7. Licorice control medicinal material; 8. Ginseng control medicinal material;

[0110] 9. Bupleurum reference medicinal material; 10. Elution with 10% ethanol; 11. Elution with 20% ethanol; 12. Elution with 30% ethanol;

[0111]...

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Abstract

The invention relates to a thin-layer identification method for Xiao Bupleurum preparations, belonging to the technical field of detection of traditional Chinese medicine preparations. The identification method comprises the following steps: preparation of the test sample: take the test sample of Xiaobupleurum preparation, add water to dissolve it, load it on a C18 solid-phase extraction column, and extract the solution of the test sample for future use; preparation of the reference substance: take the licorice , Scutellaria baicalensis, ginseng and Bupleurum as reference medicinal materials, adding water as a solvent for extraction, and loading the extract to a C18 solid-phase extraction column, and extracting the reference medicinal material solution for later use; thin-layer development: draw the above-mentioned test solution respectively Spot on the silica gel thin-layer plate and develop with developer; color development inspection: dry the above-mentioned thin-layer plate, perform color development and inspection, and compare and identify the spots of the test product and the spots of the reference medicinal material. The method uses one plate and multiple tests to conduct TLC identification of Scutellaria baicalensis, licorice, ginseng and Bupleurum in Xiaochaihu granules, and the reproducibility is good, which can be used as the identification method of this variety.

Description

technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicine preparations, in particular to a thin-layer identification method for Xiao Bupleurum preparations. Background technique [0002] Xiao Chaihu Decoction is an ancient famous prescription in "Treatise on Febrile and Miscellaneous Diseases" written by Zhang Zhongjing in the Eastern Han Dynasty. It is the representative of reconciling Shaoyang prescriptions. It has the functions of relieving exterior heat and cooling the liver, soothing the liver and stomach. [0003] The 2020 edition of the "Chinese Pharmacopoeia" contains Xiaochaihu Tablets, Xiaochaihu Effervescent Tablets, Xiaochaihu Capsules and Xiaochaihu Granules developed by Xiaochaihu Decoction using current pharmaceutical technology. Among them, Xiaochaihu Granules are the most used. Under the identification item of Xiaochaihu granules, three TLC methods were used to qualitatively identify Scutellaria baicale...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 龙飞杨亮
Owner 广东志道医药科技有限公司
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